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Team:Warsaw/Calendar-Main/21 October 2008
From 2008.igem.org
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<li>Control <a href=https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest>digest</a> of isolated plasmids with EcoRI and PstI (Orange buffer). Gel electrophoresis - proper clones found.</li></ol> | <li>Control <a href=https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest>digest</a> of isolated plasmids with EcoRI and PstI (Orange buffer). Gel electrophoresis - proper clones found.</li></ol> | ||
| - | + | <h3>Preparation of <a href=http://partsregistry.org/Part:BBa_K103002>AID under pBAD/araC (BBa_K103002)</a></h3> | |
| - | <h3>Preparation of | + | |
<h4>Michał K.</h4> | <h4>Michał K.</h4> | ||
<ol><li> Inoculation of few more colonies from plate with ligation of <a href=http://partsregistry.org/wiki/index.php?title=Part:pSB1A3>pSB1A3</a> + AraC+pBAD+AID to LB with ampicillin. </li> | <ol><li> Inoculation of few more colonies from plate with ligation of <a href=http://partsregistry.org/wiki/index.php?title=Part:pSB1A3>pSB1A3</a> + AraC+pBAD+AID to LB with ampicillin. </li> | ||
Revision as of 20:28, 27 October 2008
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Preparation of OmpA-linker-omega-linker (BBa_K103016)PiotrInoculation of few more colonies from plate with ligation of pACYC177 + OmpA-linker-omega-linker (BBa_K103016) (from 16 October) to LB with kanamycin. Preparation of alpha_linker under PT7 (BBa_K103019)Michał K.
Preparation of AID under pBAD/araC (BBa_K103002)Michał K.
Preparation of OmpA-linker-alpha-linkerMichał K.Inoculation of colonies from plate with ligation (OmpA_alpha_) to LB with kanamycin. |
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