Team:PennState/diauxie/progress

From 2008.igem.org

(Difference between revisions)
m (left nav integration)
Line 133: Line 133:
<!-- First table displays centered iGEM 2007 Logo -->   
<!-- First table displays centered iGEM 2007 Logo -->   
<div id="header">
<div id="header">
-
  <img src="https://static.igem.org/mediawiki/2008/d/df/Penn_state_igem_logo.JPG" alt="Penn State" />
+
  <img src="https://static.igem.org/mediawiki/2008/a/ad/Penn_state_igem_logo2.JPG" alt="Penn State" />
</div>
</div>

Revision as of 15:28, 28 October 2008

Home The Team The Project Parts Notebook

Diauxie Elimination

Introduction
Implementation
Progress
Parts
References

NHR
Biosensors

Introduction
Smart Fold
Overview
Nuclear Fusion
Overview
 Progress & Results

We have completed our first set of cloning phases and are conducting our first round of testing and modification. Each test construct (promoter + GFP) has been cloned into pSB1A2 and transformed into DH5α, W3110 ∆xylB-G, and W3110 ∆xylB-R. Preliminary induction studies were run to find the optimal induction time and to see in what range OD and fluorescence were linearly related.

Promoter pSB1A2 DH5α W3110 ΔxylB-G W3110 ΔxylB-R
PN
P1
P3
P5

Test were then run to compare the level of induction between mixes of xylose, glucose and xylose, and glucose. The goal of this project is to obtain a noticeably higher level of induction when induced with xylose and glucose compared to the wildtype construct.

Retrieved from "http://2008.igem.org/Team:PennState/diauxie/progress"