Team:Warsaw/Calendar-Main/21 October 2008

From 2008.igem.org

(Difference between revisions)
Line 18: Line 18:
<li>Control <a href=https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest>digest</a> of isolated plasmids with EcoRI and PstI (Orange buffer). Gel electrophoresis - proper clones found.</li></ol>
<li>Control <a href=https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest>digest</a> of isolated plasmids with EcoRI and PstI (Orange buffer). Gel electrophoresis - proper clones found.</li></ol>
 +
 +
<a name="fig1"><img src="https://static.igem.org/mediawiki/2008/1/11/Traw2_19_10_2008.jpg"></a>
 +
<var><b>Fig. 19.</b> Traw2_19_10_2008.jpg</var>
 +
 +
 +
<h3>Preparation of <a href=http://partsregistry.org/Part:BBa_K103002>AID under pBAD/araC (BBa_K103002)</a></h3>
<h3>Preparation of <a href=http://partsregistry.org/Part:BBa_K103002>AID under pBAD/araC (BBa_K103002)</a></h3>

Revision as of 23:52, 27 October 2008

Gallery Bricks Notebook Team Project Home


Previous day
return to main notebook page
Previous entry
Interactive list of topics
next notebook entry

 



Preparation of OmpA-linker-omega-linker (BBa_K103016)

Piotr

Inoculation of few more colonies from plate with ligation of pACYC177 + OmpA-linker-omega-linker (BBa_K103016) (from 16 October) to LB with kanamycin.

Preparation of alpha_linker under PT7 (BBa_K103019)

Michał K.

  1. Isolation of plasmids from cultures inoculated on previous day (pSB2K3 + alpha_linker under PT7 (BBa_K103019)).
  2. Control digest of isolated plasmids with EcoRI and PstI (Orange buffer). Gel electrophoresis - proper clones found.
Fig. 19. Traw2_19_10_2008.jpg

Preparation of AID under pBAD/araC (BBa_K103002)

Michał K.

  1. Inoculation of few more colonies from plate with ligation of pSB1A3 + AID under pBAD/araC (BBa_K103002) to LB with ampicillin.
  2. Isolation of plasmids from cultures inoculated on previous day - pSB1A3 +AID under pBAD/araC (BBa_K103002).
  3. Control digest of isolated plasmids with EcoRI and PstI (Orange buffer). Gel electrophoresis - still no proper clones found.

Preparation of OmpA_linker_alpha_linker (BBa_K103014)

Michał K.

Inoculation of colonies from plate with ligation (OmpA_alpha_) to LB with kanamycin.

Retrieved from "http://2008.igem.org/Team:Warsaw/Calendar-Main/21_October_2008"