Team:Edinburgh/Results

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Revision as of 22:39, 27 October 2008

Edinburgh iGEM 2008

Results and current status as of 29 October 2008. We are hoping to obtain some last-minute results to present at the Jamboree.

Overview

Our results are promising but our dream of using bacteria to convert cellulose into starch and β-carotene is still unrealised. Fortunately, the work will be continued by two undergraduate students after Christmas.

Cellulolysis device

cenA (endoglucanase) and cex (exoglucanase): Biobricks made, but encountered problems when adding ribosome binding site.
bglX (beta glucosidase): Biobrick made.
P_cstA (promoter): Biobrick made and tested - successful.
- P_cstA promoter assay with xylE as repoter

Glycogenesis device

glgC and glgC16 (ADP-glucose pyrophosphorylase, responsible for rate limiting step in glycogenesis): Biobricks made and tested - successful.

Starch synthesis device

su1 and iso2 (isoamylases): Work in progress - to be continued in an undergraduate honours project.

Lycopene generator

dxs+crtE+crtB+crtI+crtY: Work in progress - encountered problem at final step.

Limonene synthase device

dxs+LIMS+appY: Biobrick made - still being tested (last test failed to detect limonene synthesis).

Acknowledgements

We would like to thank the following researchers for their contributions to our project:

Raman spectroscopy for glycogen production was done by Dr Rabah Mouras.
Mutagenesis of crtI was done by Douglas Armstrong.
The maize isoamylase genes su1 and iso2 were generously donated by Professor Alan Myers of Iowa State University.

@@ Line 19: / Line 19: @@
 ** [[Team:Edinburgh/Results/Glycogen2|Glycogen Assay 2 (Qualitative: Iodine)]]
 ** [[Team:Edinburgh/Results/Glycogen3|Glycogen Assay 3 (Quantitative: Raman)]]
-** [[Team:Edinburgh/Results/Glycogen_4|Glycogen Assay 4 (Quantitative: Raman)]]
+** [[Team:Edinburgh/Results/Glycogen4|Glycogen Assay 4 (Quantitative: Raman)]]
 == Starch synthesis device ==