Team:Warsaw/Calendar-Main/16 October 2008

From 2008.igem.org

(Difference between revisions)
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<h4>Michał K.</h4>
<h4>Michał K.</h4>
<ol><li><a href=https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation>Isolation</a> of plasmids from culture inoculated on previous day (<a href=http://partsregistry.org/wiki/index.php?title=Part:pSB1A3>pSB1A3</a> + <a href=http://partsregistry.org/Part:BBa_K103002>AID under pBAD/araC (BBa_K103002)</a>).</li>
<ol><li><a href=https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation>Isolation</a> of plasmids from culture inoculated on previous day (<a href=http://partsregistry.org/wiki/index.php?title=Part:pSB1A3>pSB1A3</a> + <a href=http://partsregistry.org/Part:BBa_K103002>AID under pBAD/araC (BBa_K103002)</a>).</li>
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<li>Control <a href=https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest>digest</a> of isolated plasmids with EcoRI and PstI (Orange buffer). Gel electrophoresis - still no proper clones found.</li>
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<li>Control <a href=https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest>digest</a> of isolated plasmids with EcoRI and PstI (Orange buffer). Gel electrophoresis - still no proper clones found <a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/16_October_2008#fig1">Fig. 1</a>.</li>
<li> Repetition of overnight <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">ligation</a> of isolated DNA fragments: <a href=http://partsregistry.org/wiki/index.php?title=Part:pSB1A3>pSB1A3</a> + <a href=http://partsregistry.org/Part:BBa_K103002>AID under pBAD/araC (BBa_K103002)</a>.</li></ol>
<li> Repetition of overnight <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">ligation</a> of isolated DNA fragments: <a href=http://partsregistry.org/wiki/index.php?title=Part:pSB1A3>pSB1A3</a> + <a href=http://partsregistry.org/Part:BBa_K103002>AID under pBAD/araC (BBa_K103002)</a>.</li></ol>
<a name="fig1"><img src="https://static.igem.org/mediawiki/2008/3/3c/Traw2_15_10_2008.jpg"></a>
<a name="fig1"><img src="https://static.igem.org/mediawiki/2008/3/3c/Traw2_15_10_2008.jpg"></a>
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<var><b>Fig. 18.</b>Traw2_15_10_2008.jpg </var>
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<var><b>Fig. 18.</b>EcoRI/PstI digests of isolated plasmids (pSB1A3+AID under pBAD/araC (BBa_K103002)<br>
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1. Marker<br>
 +
2-9. EcoRI/PstI digests of isolated plasmids<br></var>
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<ol>
<ol>
<li><a href=https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation>Isolation</a> of plasmids from culture inoculated on previous day - <a href=http://partsregistry.org/Part:pSB2K3>pSB2K3</a> + <a href=http://partsregistry.org/Part:BBa_K103017>OmpA_linker_alpha_linker under Plac (BBa_K103017)</a></li>
<li><a href=https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation>Isolation</a> of plasmids from culture inoculated on previous day - <a href=http://partsregistry.org/Part:pSB2K3>pSB2K3</a> + <a href=http://partsregistry.org/Part:BBa_K103017>OmpA_linker_alpha_linker under Plac (BBa_K103017)</a></li>
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<li>Control <a href=https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest>digest</a> of isolated plasmids with EcoRI and PstI (Orange buffer). Gel electrophoresis - we found good clones.</li>
+
<li>Control <a href=https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest>digest</a> of isolated plasmids with EcoRI and PstI (Orange buffer). Gel electrophoresis - we found good clones <a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/16_October_2008#fig2">Fig. 2</a>.</li>
</ol>
</ol>
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<a name="fig1"><img src="https://static.igem.org/mediawiki/2008/f/f7/Traw_16_10_2008.jpg"></a>
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<a name="fig2"><img src="https://static.igem.org/mediawiki/2008/f/f7/Traw_16_10_2008.jpg"></a>
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<var><b>Fig. 25.</b>traw_16_10_2008.jpg </var>
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<var><b>Fig. 2.</b>EcoRI/PstI digests of isolated plasmids<br>
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1. Marker<br>
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2-7. digests of pSB1A3 carrying alpha_linker under PT7 (BBa_K103019)<br>
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8-11. digests of plasmid carrying alpha_linker under pLac<br>
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11-17. digests of plasmid carrying linker_alpha<br></var>

Revision as of 14:25, 28 October 2008

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Preparation of linker_alpha (BBa_K103009)

Michał K.

  1. Isolation of plasmids from culture inoculated on previous day.
  2. Control digest of isolated plasmids with EcoRI and PstI (Orange buffer). Gel electrophoresis - proper clones found.

Preparation of OmpA-linker-omega-linker (BBa_K103016)

Michał K.

Inoculation of colonies from plate with new ligation of pACYC177 + OmpA-linker-omega-linker (BBa_K103016) to LB with kanamycin.

Preparation of alpha_linker under PT7 (BBa_K103019)

Michał K.

  1. Control digest of isolated plasmid - pSB1A3 carrying alpha_linker under PT7 (BBa_K103019) fragment (14 October) with EcoRI and PstI (Orange buffer). Gel electrophoresis - proper clones found.
  2. Overnight digest pSB1A3 carrying alpha_linker under PT7 (BBa_K103019) with EcoRI and BcuI (BamHI buffer).

Preparation of AID under pBAD/araC (BBa_K103002)

Michał K.

  1. Isolation of plasmids from culture inoculated on previous day (pSB1A3 + AID under pBAD/araC (BBa_K103002)).
  2. Control digest of isolated plasmids with EcoRI and PstI (Orange buffer). Gel electrophoresis - still no proper clones found Fig. 1.
  3. Repetition of overnight ligation of isolated DNA fragments: pSB1A3 + AID under pBAD/araC (BBa_K103002).
Fig. 18.EcoRI/PstI digests of isolated plasmids (pSB1A3+AID under pBAD/araC (BBa_K103002)
1. Marker
2-9. EcoRI/PstI digests of isolated plasmids

Preparation of OmpA_linker_alpha_linker under Plac (BBa_K103017)

Michał K.

  1. Isolation of plasmids from culture inoculated on previous day - pSB2K3 + OmpA_linker_alpha_linker under Plac (BBa_K103017)
  2. Control digest of isolated plasmids with EcoRI and PstI (Orange buffer). Gel electrophoresis - we found good clones Fig. 2.
Fig. 2.EcoRI/PstI digests of isolated plasmids
1. Marker
2-7. digests of pSB1A3 carrying alpha_linker under PT7 (BBa_K103019)
8-11. digests of plasmid carrying alpha_linker under pLac
11-17. digests of plasmid carrying linker_alpha

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