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Team:NTU-Singapore/Notebook/20 June 2008
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(Difference between revisions)
(New page: =Friday 20 June= ==Morning:== *Miniprep for pLacI, followed by DNA concentrating using QiAQuick PCR purification kit. ==Afternoon:== *Transformation and cell cloning 4 empty plasmids from ...) |
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**[http://partsregistry.org/wiki/index.php?title=Part:pSB1AK3 pSB1AK3] | **[http://partsregistry.org/wiki/index.php?title=Part:pSB1AK3 pSB1AK3] | ||
**[http://partsregistry.org/wiki/index.php?title=Part:pSB1AT3 pSB1AT3] | **[http://partsregistry.org/wiki/index.php?title=Part:pSB1AT3 pSB1AT3] | ||
| + | *Transformation and cloning of LacI-GFP into LuxS knockout bacteria. | ||
*Make LsrA competent cells. Then transform 0.3 ul LacI-GFP plasmid into these cells. | *Make LsrA competent cells. Then transform 0.3 ul LacI-GFP plasmid into these cells. | ||
| + | |||
==Evening:== | ==Evening:== | ||
*Gel extraction (using QiAQuick Gel extraction Kit)==>Ligation (using T4 DNA ligase) ==> Transformation for the following 4 samples: | *Gel extraction (using QiAQuick Gel extraction Kit)==>Ligation (using T4 DNA ligase) ==> Transformation for the following 4 samples: | ||
Revision as of 08:45, 23 June 2008
Contents |
Friday 20 June
Morning:
- Miniprep for pLacI, followed by DNA concentrating using QiAQuick PCR purification kit.
Afternoon:
- Transformation and cell cloning 4 empty plasmids from Biobrick registry:
- [http://partsregistry.org/wiki/index.php?title=Part:pSB1A3 pSB1A3]
- [http://partsregistry.org/wiki/index.php?title=Part:pSB1AC3 pSB1AC3]
- [http://partsregistry.org/wiki/index.php?title=Part:pSB1AK3 pSB1AK3]
- [http://partsregistry.org/wiki/index.php?title=Part:pSB1AT3 pSB1AT3]
- Transformation and cloning of LacI-GFP into LuxS knockout bacteria.
- Make LsrA competent cells. Then transform 0.3 ul LacI-GFP plasmid into these cells.
Evening:
- Gel extraction (using QiAQuick Gel extraction Kit)==>Ligation (using T4 DNA ligase) ==> Transformation for the following 4 samples:
- E7 Insert - Empty plasmid vector (from GFP)
- SupD Insert - Empty plasmid vector (from GFP)
- T7ptag Insert - Empty plasmid vector (from GFP)
- GFP insert - pFE vector (for characterization of Fe promoter)
