"
Team:NTU-Singapore/Notebook/23 June 2008
From 2008.igem.org
(Difference between revisions)
(New page: =Monday 23 June= ==Morning (9am-1230pm) *DNA extraction (using Miniprep kit) for 21 samples that Choon Kit inoculated on [[https://2008.igem.org/wiki/index.php?title=Team:NTU-Singapore/Note...) |
m |
||
| Line 1: | Line 1: | ||
=Monday 23 June= | =Monday 23 June= | ||
| - | ==Morning (9am-1230pm) | + | ==Morning (9am-1230pm)== |
| - | *DNA extraction (using Miniprep kit) for 21 samples that Choon Kit inoculated on | + | *DNA extraction (using Miniprep kit) for 21 samples that Choon Kit inoculated on [https://2008.igem.org/wiki/index.php?title=Team:NTU-Singapore/Notebook/22_June_2008 Sunday] |
*Digestion of the above 21 plasmids by EcoRI and PstI, followed by Gel electrophoresis check after 2 hours incubation. | *Digestion of the above 21 plasmids by EcoRI and PstI, followed by Gel electrophoresis check after 2 hours incubation. | ||
*Digestion LacI promoter insert using SpeI and EcoRI sequentially with QiAgen PCR purification kit in between. | *Digestion LacI promoter insert using SpeI and EcoRI sequentially with QiAgen PCR purification kit in between. | ||
*Digestion of RBS 0034 vector using XbaI and EcoRI sequentially and QIAgen PCR purification kit. | *Digestion of RBS 0034 vector using XbaI and EcoRI sequentially and QIAgen PCR purification kit. | ||
Revision as of 08:57, 23 June 2008
Monday 23 June
Morning (9am-1230pm)
- DNA extraction (using Miniprep kit) for 21 samples that Choon Kit inoculated on Sunday
- Digestion of the above 21 plasmids by EcoRI and PstI, followed by Gel electrophoresis check after 2 hours incubation.
- Digestion LacI promoter insert using SpeI and EcoRI sequentially with QiAgen PCR purification kit in between.
- Digestion of RBS 0034 vector using XbaI and EcoRI sequentially and QIAgen PCR purification kit.
