Team:Imperial College/Major Results/B.subtilis
From 2008.igem.org
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{{Imperial/Box1|''B. subtilis'' Biobricks|In order to pursue our project we had to design and build a variety of biobricks parts for ''B. subtilis''. These can be split up into three groups: | {{Imperial/Box1|''B. subtilis'' Biobricks|In order to pursue our project we had to design and build a variety of biobricks parts for ''B. subtilis''. These can be split up into three groups: | ||
*'''Promoter and Ribosome Binding Sites (RBS)''' - These promoter and RBS combinations include a number of inducible and constitutive promoters combined with weak and strong ribosome binding sites. | *'''Promoter and Ribosome Binding Sites (RBS)''' - These promoter and RBS combinations include a number of inducible and constitutive promoters combined with weak and strong ribosome binding sites. | ||
- | *'''Coding Regions''' - These parts include specific coding regions concerned with our project such as biomaterials and more general coding regions such as antibiotic resistance genes | + | *'''Coding Regions''' - These parts include specific coding regions concerned with our project such as biomaterials, and more general coding regions such as antibiotic resistance genes that can be used for selection markers. |
*'''Integration Sites''' - As an alternative to maintaining genetic devices on extra-chromosomal plasmids we investigated the construction of parts to allow integration of genetic devices into the ''B. subtilis'' chromosome. | *'''Integration Sites''' - As an alternative to maintaining genetic devices on extra-chromosomal plasmids we investigated the construction of parts to allow integration of genetic devices into the ''B. subtilis'' chromosome. | ||
|[[Image:B.subtilis parts.PNG|right|300px]] | |[[Image:B.subtilis parts.PNG|right|300px]] |
Revision as of 15:36, 28 October 2008
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