/Validation
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(New page: {{Imperial/StartPage2}} = Validating Tracking Software = ImageJ was used primarily to analyse images. Various plugins were tested, such as SpotTracker and Particle Tracker, but both faile...) |
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|'''Error in Y Coordinate''' | |'''Error in Y Coordinate''' | ||
|- | |- | ||
- | |[[Image:Multiple Error X. | + | |[[Image:Multiple Error X.jpg|300px]] |
- | |[[Image:Multiple Error Y px. | + | |[[Image:Multiple Error Y px.jpg|300px]] |
|- | |- | ||
|Mean = 1.7354, Standard Deviation = 1.7187 | |Mean = 1.7354, Standard Deviation = 1.7187 | ||
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|'''Error in Distance''' | |'''Error in Distance''' | ||
|- | |- | ||
- | |[[Image:Multiple Error X Displacement. | + | |[[Image:Multiple Error X Displacement.jpg|300px]] |
- | |[[Image:Multiple Error Y Displacement. | + | |[[Image:Multiple Error Y Displacement.jpg|300px]] |
- | |[[Image:Multiple_Error_Distance. | + | |[[Image:Multiple_Error_Distance.jpg|300px]] |
|- | |- | ||
|Mean = -0.0022, Standard Deviation = 1.0002 | |Mean = -0.0022, Standard Deviation = 1.0002 | ||
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|'''Error in Y Coordinate''' | |'''Error in Y Coordinate''' | ||
|- | |- | ||
- | |[[Image:Volocity X Error. | + | |[[Image:Volocity X Error.jpg|300px]] |
- | |[[Image:Volocity Y Error. | + | |[[Image:Volocity Y Error.jpg|300px]] |
|- | |- | ||
|Mean = -1.7149, Standard Deviation = 0.1376 | |Mean = -1.7149, Standard Deviation = 0.1376 | ||
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|'''Error in Distance''' | |'''Error in Distance''' | ||
|- | |- | ||
- | |[[Image:Volocity X Displacement Error. | + | |[[Image:Volocity X Displacement Error.jpg|300px]] |
- | |[[Image:Volocity Y Displacement Error. | + | |[[Image:Volocity Y Displacement Error.jpg|300px]] |
- | |[[Image:Volocity Distance Error. | + | |[[Image:Volocity Distance Error.jpg|300px]] |
|- | |- | ||
|Mean = 8.2732e-005, Standard Deviation = 0.1111 | |Mean = 8.2732e-005, Standard Deviation = 0.1111 |
Revision as of 17:30, 28 October 2008
Validating Tracking SoftwareImageJ was used primarily to analyse images. Various plugins were tested, such as SpotTracker and Particle Tracker, but both failed to producing any form of convincing result. Microscopy videos were captured using Volocity software, which also provided a cell tracking function. Thus analysis of synthetic videos was done manually and using 64-Bit Volocity analysis software. The synthetic video shown to the right was used as an input for the tracking of synthetic cells. VolocityVolocity 64-Bit was used to analyse synthetic videos created. The following protocol was followed:
Manual TrackingSynthetic cells were tracked using ImageJ via the Manual Tracking Plugin. The following protocol was followed:
Validation ProcessA synthetic video of five cells with changing shape was used in the evaluation of tracking software. Each cell was tracked using respective tracking software, and their coordinates in pixels were obtained. The following steps were taken to analyse tracking data:
The errors associated with manual tracking and Volocity software are shown below. All errors are given in terms of pixels. Manual Tracking
Volocity
Evaluation
Conclusion
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