Team:MIT

From 2008.igem.org

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Revision as of 14:58, 24 June 2008

Welcome to the MIT team Wiki for iGEM 2008

iGEM is the international genetically engineered machines competition.
The objective of the competition is to design and build an engineered biological system using [http://en.wikipedia.org/wiki/DNA DNA].
To see examples of the amazing possibilities of iGEM, check out last years [http://parts.mit.edu/igem07/index.php/Main_Page iGEM page]
Read our promotional brochure to learn about synthetic biology at MIT (front and back).
iGEM at MIT is possible because of outside support, contact Tom Knight (tk [at] mit.edu) to help out!

Resources

Interested in learning more about iGEM 2008? Explore below for an example of what's possible.

For more info on this year's iGEM please see 2008.igem.org.

Many more valuable links and helpful tidbits are available at the [http://parts.mit.edu/wiki/index.php/Resources iGEM Resources page]

For visitors

Read more about engineering biology.
[http://stellar.mit.edu/S/course/20/sp08/20.020/ 20.020: MIT's introduction to bioengineering design]

External links

iGEM on OpenWetWare
[http://parts.mit.edu/registry BioBricks Parts registry]
[http://parts.mit.edu/igem07/index.php/Main_Page Official iGEM 2007 wiki]
[http://parts.mit.edu/igem Official iGEM 2006 wiki]
[http://openwetware.org/wiki/IGEM:MIT/2007 MIT 2007 Wiki]
[http://parts.mit.edu/wiki/index.php/MIT_2006 MIT 2006 Wiki]
BioBricks parts contributed by MIT:
- [http://parts.mit.edu/registry/index.php/Part:BBa_I728005 OmpC Surface Display]
- [http://parts.mit.edu/registry/index.php/Part:BBa_I728004 CPX Surface Display]
[http://openwetware.org/wiki/20.109(S08) 20.109 instructions (2008)]

Internal links

[[../2008/Notebook/Yogurt|Team Notebook]]

Protocols

[http://openwetware.org/wiki/Endy:DNA_ligation_using_T4_DNA_ligase T4 ligation]
[http://openwetware.org/wiki/TOP10_chemically_competent_cells Top10 chem competent cell transformation]
[http://web.mit.edu/biopolymers/www/DNA.html DNA sequencing]
[http://openwetware.org/wiki/Endy_pcr PCR]
[http://openwetware.org/wiki/Knight:Restriction_Digest Restriction Digests]

Good Lab Citizenship

Go over the Do's and Dont's of Good Lab Citizenship

Wiki editing resources

Simple wiki editing examples
[http://en.wikipedia.org/wiki/Wikipedia:How_to_edit_a_page How to edit a page (extended - wikipedia)]
[http://www.mediawiki.org/wiki/Help:Images Help with images (wikipedia)]
[http://en.wikipedia.org/wiki/Help:Table Help with tables (wikipedia)]

Information

Want to help out the iGEM team?

iGEM at MIT is partly supported by the [http://web.mit.edu/urop/basicinfo/ Undergraduate Research Opportunity Program] and faculty including Drew Endy and Tom Knight.
We guarantee UROP funding for undergrads. We need help to continue supporting undergrads, paying registration fees, and supplying lab reagents, contact Tom Knight to help out!
Read more about our team on our fundraising notebook.

Want to join the iGEM team?

Graduate students interested in advising the team should email grads [AT] igem.mit.edu
Undergraduate students should get in touch during IAP or Spring of 2009!

Schedule

April 10 - UROP funding deadline
April 18 - iGEM registration opens
May 3 - Teachers Workshop, MIT, USA
May 9 - Registration closes
June 9 - Summer term, lab work begins
June 15 - Team rosters due
July 1 - Registration fee due
August 1 - Team project descriptions due
August 19 - Summer term ends
September 1 - Final team roster due
October 1 - Jamboree attendance fees due
October 15 - Project Summary form due
October 29 - Project and part documentation due
October 29 - BioBrick Part DNA received by the Registry
November 8-9 - iGEM competition Jamboree, MIT, USA

Brainstorming

Brainstorming

Meeting Logistics

People

The MIT iGEM team consists of six undergraduate students working full-time during summer 2008 on engineering a biological system. In addition, we have a number of graduate student and faculty advisers.

Picture of MIT iGEM 2008 (coming soon!)

Students

Grad Advisors

Faculty Advisors

Email grads: grads [AT] igem.mit.edu

Email undergrads: team [AT] igem.mit.edu

alternative address: igem2008-all [AT] mit.edu

* Our goal is to engineer a common yogurt bacteria, Lactobacillus bulgaricus, so that it will express the 20aa peptide p1025. A clinical study (Kelly CG et al.; Nature Biotechnol. 1999) reports that p1025 is good for your teeth. p1025 reduces oral colonization of Streptococcus mutans, a tooth-decaying bacterium.

   *
         o Stage 1: Construction of p1025 fusion peptide and expression of gene in E. coli. This is an intermediate step to evaluate gene function and protein secretion/efficacy.
         o Stage 2: Binding Assay - see if the p1025 produced by E.coli inhibits binding of S. mutants to hydroxyapatite (HA) beads.
         o Stage 3: Expression of p1025 in Lactobacillus.
         o Stage 4: Make yogurt with modified Lactobacillus and test for inhibition of S. mutants binding.

Your team picture

Team Example 2

Home	The Team	The Project	Parts Submitted to the Registry	Modeling	Notebook

(Or you can choose different headings. But you must have a team page, a project page, and a notebook page.)

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 {|
 | rowspan=2 | [[Image:team-logo.jpg|218px]]
-[[Image:Mit-blackred-header2.gif|194px http://web.mit.edu]]
+|[[Image:Teambiogurt.jpg|200px|right|frame]]
 |
 '''Welcome to the MIT team Wiki for iGEM 2008'''