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Template:Team:UC Berkeley/Notebook/CC construction
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CgATAgaattcATGagatcttacccatacgacgtcccagac | CgATAgaattcATGagatcttacccatacgacgtcccagac | ||
| - | + | cc003 Reverse biobricking of {<HA!} | |
ccagtggatccttaccagcgtagtctgggacgtcgtatgggtaag | ccagtggatccttaccagcgtagtctgggacgtcgtatgggtaag | ||
</pre> | </pre> | ||
| Line 43: | Line 43: | ||
cc005 Reverse Biobricking of {<myc>} | cc005 Reverse Biobricking of {<myc>} | ||
ccagtggatccCAGATCCTCTTCTGAGATGAGTTTTTG | ccagtggatccCAGATCCTCTTCTGAGATGAGTTTTTG | ||
| + | </pre> | ||
| + | |||
| + | ==K112504== | ||
| + | <pre> | ||
| + | Construction of {<myc!} basic part K112503 | ||
| + | |||
| + | Wobble PCR of cc004/cc006 (___bp, EcoRI/BamHI) | ||
| + | Sub into pBca1256 (EcoRI/BamHI) | ||
| + | Product is pBca1256-K112504 | ||
| + | -------------------------------------------- | ||
| + | cc004 Forward Biobricking of {<myc>} | ||
| + | CgATAgaattcATGagatctGAACAAAAACTCATCTCAGAAGAGG | ||
| + | cc006 Reverse Biobricking of {<myc!} | ||
| + | ccagtggatccTTACAGATCCTCTTCTGAGATGAGTTTTTGTTC | ||
</pre> | </pre> | ||
Revision as of 15:38, 24 June 2008
Contents |
K112501
Construction of {<HA>} basic part K112501
Wobble PCR of cc001/cc002 (___bp, EcoRI/BamHI)
Sub into pBca1256 (EcoRI/BamHI)
Product is pBca1256-K112501
--------------------------------------------
cc001 Forward Biobricking of {<HA>}
CgATAgaattcATGagatcttacccatacgacgtcccagac
cc002 Reverse biobricking of {<HA>}
ccagtggatccccagcgtagtctgggacgtcgtatggg
K112502
Construction of {<HA!} basic part K112502
Wobble PCR of cc001/cc003 (___bp, EcoRI/BamHI)
Sub into pBca1256 (EcoRI/BamHI)
Product is pBca1256-K112502
--------------------------------------------
cc001 Forward Biobricking of {<HA>}
CgATAgaattcATGagatcttacccatacgacgtcccagac
cc003 Reverse biobricking of {<HA!}
ccagtggatccttaccagcgtagtctgggacgtcgtatgggtaag
K112503
Construction of {<myc>} basic part K112503
Wobble PCR of cc004/cc005 (___bp, EcoRI/BamHI)
Sub into pBca1256 (EcoRI/BamHI)
Product is pBca1256-K112503
--------------------------------------------
cc004 Forward Biobricking of {<myc>}
CgATAgaattcATGagatctGAACAAAAACTCATCTCAGAAGAGG
cc005 Reverse Biobricking of {<myc>}
ccagtggatccCAGATCCTCTTCTGAGATGAGTTTTTG
K112504
Construction of {<myc!} basic part K112503
Wobble PCR of cc004/cc006 (___bp, EcoRI/BamHI)
Sub into pBca1256 (EcoRI/BamHI)
Product is pBca1256-K112504
--------------------------------------------
cc004 Forward Biobricking of {<myc>}
CgATAgaattcATGagatctGAACAAAAACTCATCTCAGAAGAGG
cc006 Reverse Biobricking of {<myc!}
ccagtggatccTTACAGATCCTCTTCTGAGATGAGTTTTTGTTC
