Team:MIT/Notebook

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Revision as of 16:03, 24 June 2008

Notebook

150px <sitesearch>title=Search this project</sitesearch>

Customize your entry pages

Description

Our goal is to engineer a common yogurt bacteria, Lactobacillus bulgaricus, so that it will express the 20aa peptide p1025. A clinical study [http://www.ncbi.nlm.nih.gov/pubmed/9920267?dopt=Abstract (Kelly CG et al.; Nature Biotechnol. 1999)] reports that p1025 is good for your teeth. p1025 reduces oral colonization of Streptococcus mutans, a tooth-decaying bacterium.

- Stage 1: Construction of p1025 fusion peptide and expression of gene in E. coli. This is an intermediate step to evaluate gene function and protein secretion/efficacy.
- Stage 2: Binding Assay - see if the p1025 produced by E.coli inhibits binding of S. mutants to hydroxyapatite (HA) beads.
- Stage 3: Expression of p1025 in Lactobacillus.
- Stage 4: Make yogurt with modified Lactobacillus and test for inhibition of S. mutants binding.

Notes

Group meetings every Friday at 4pm in 68-474 (except 7/11 and 8/1 in 68-674)
Journal club meetings as needed every Friday at 11am in 68-474

Information

[[../Yogurt/Brainstorming |Brainstorming]]

[[../Yogurt/Notes |Daily Notebook]]

[[../Yogurt/sequences |Primers/Plasmids/PCR sequences]]

[[../Yogurt/lbulgaricus |Lactobacillus delbrueckii subsp. bulgaricus Information & Protocols]]

[[../Yogurt/toothbindingassay | Tooth binding assay protocol]]
[[../Yogurt/Vocabulary List |Vocabulary List]]

Useful Links

[http://www.biology.utah.edu/jorgensen/wayned/ape/ |DNA Plasmid Editor]
[http://slam.bs.jhmi.edu/gd/ |Peptide Sequence Manipulations]
Primer Analyzer Tool (check temperature & GC content) [http://www.idtdna.com/analyzer/Applications/OligoAnalyzer/Default.aspx | One] [http://www.idtdna.com/analyzer/Applications/OligoAnalyzer/Default.aspx | Two]
PMID 9920267
A synthetic peptide adhesion epitope as a novel antimicrobial agent.
PMID 9062560
Inhibition of Streptococcus mutans adsorption to hydroxyapatite by low-molecular-weight chitosans.
PMID 12788739
(a secretion signal sequence described; can use the promoter, RBS and secretion signal for this protein for p1025).
PMID 11772607
Electrotransformation of Lactobacillus delbrueckii subsp. bulgaricus and L. delbrueckii subsp. lactis with various plasmids.

@@ Line 1: / Line 1: @@
 ==Notebook==
+{|{{table}} width="800"
+|-
+|style="background-color: #cdde95;" align="center"|
+<!-- ## START calendar column -->
+<LNCalendar></LNCalendar>
+<!-- ## END calendar column -->
+|align="center" style="background-color: #e5edc8;" |
+<!-- ## START search column: Place your logo here. Try keep it below 200px in width and 150px in height. ##  -->
+[[Image:igem-logo-150px.png|150px]] <sitesearch>title=Search this project</sitesearch>
+<!-- ## END search column  ## -->
+|-
+|colspan="2" style="background-color: #F2F2F2;" align="right"|[[/Entry_Base|Customize your entry pages]] [[Help:Notebook/Project_Base/Customize_entry_page|<html><img src="/images/a/aa/Help.png" border="0" /></html>]]
+|-
+|colspan="2"|
+==Description==
-You should make use of the calendar feature on the wiki and start a lab notebook.  This may be looked at by the judges to see how your work progressed throughout the summer.  It is a very useful organizational tool as well.
+*Our goal is to engineer a common yogurt bacteria, Lactobacillus bulgaricus, so that it will express the 20aa peptide p1025. A clinical study [http://www.ncbi.nlm.nih.gov/pubmed/9920267?dopt=Abstract (Kelly CG et al.; Nature Biotechnol. 1999)] reports that p1025 is good for your teeth. p1025 reduces oral colonization of ''Streptococcus mutans'', a tooth-decaying bacterium.
-Find more information on how to use the calendar feature by going to the [[Help:Calendar | general calendar page]].
+** Stage 1: Construction of p1025 fusion peptide and expression of gene in E. coli. This is an intermediate step to evaluate gene function and protein secretion/efficacy.
+** Stage 2: Binding Assay - see if the p1025 produced by E.coli inhibits binding of ''S. mutants'' to hydroxyapatite (HA) beads.
+** Stage 3: Expression of p1025 in Lactobacillus.
+** Stage 4: Make yogurt with modified Lactobacillus and test for inhibition of ''S. mutants'' binding.
+==Notes==
+* Group meetings every Friday at 4pm in 68-474 (except 7/11 and 8/1 in 68-674)
+* Journal club meetings as needed every Friday at 11am in 68-474
+==Information==
+* [[../Yogurt/Brainstorming |Brainstorming]]
+* [[../Yogurt/Notes |Daily Notebook]]
+* [[../Yogurt/sequences |Primers/Plasmids/PCR sequences]]
+* [[../Yogurt/lbulgaricus |''Lactobacillus delbrueckii'' subsp. ''bulgaricus'' Information & Protocols]]
+* [[../Yogurt/toothbindingassay | Tooth binding assay protocol]]
+* [[../Yogurt/Vocabulary List |Vocabulary List]]
+==Useful Links==
+*[http://www.biology.utah.edu/jorgensen/wayned/ape/ |DNA Plasmid Editor]
+*[http://slam.bs.jhmi.edu/gd/ |Peptide Sequence Manipulations]
+*Primer Analyzer Tool (check temperature & GC content) [http://www.idtdna.com/analyzer/Applications/OligoAnalyzer/Default.aspx | One] [http://www.idtdna.com/analyzer/Applications/OligoAnalyzer/Default.aspx | Two]
+*PMID 9920267<br style="clear:both;"/> A synthetic peptide adhesion epitope as a novel antimicrobial agent.<br style="clear:both;"/>
+*PMID 9062560<br style="clear:both;"/> Inhibition of Streptococcus mutans adsorption to hydroxyapatite by low-molecular-weight chitosans.<br style="clear:both;"/>
+*PMID 12788739<br style="clear:both;"/> (a secretion signal sequence described; can use the promoter, RBS and secretion signal for this protein for p1025).<br style="clear:both;"/>
+*PMID 11772607<br style="clear:both;"/> Electrotransformation of Lactobacillus delbrueckii subsp. bulgaricus and L. delbrueckii subsp. lactis with various plasmids.
+<!--|-
+|colspan="2" style="background-color: #F2F2F2;"|
+{{LnNotebookRecentChanges2}}
+|} -->
+__NOEDITSECTION__
+__NOTOC__