Template:Team:UC Berkeley/Notebook/SC construction

From 2008.igem.org

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pBca1256-K112000
 
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PCR bx1/bx2 on Bjh1301 (688 bp, EcoRI/BamHI/DpnI)
 
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Sub into pBca1256 (EcoRI/BamHI)
 
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Product is pBca1256-K112000
 
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Forward Biobricking of {T4 Holin!}
 
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ccagtGAATTCatgAGATCTatggcagcacctagaatatc
 
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Reverse Biobricking of {T4 Holin!}
 
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cgttaGGATCCttatttagcccttcctaatattctggccgcc
 

Revision as of 18:40, 24 June 2008