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Template:Team:UC Berkeley/Notebook/SC construction
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| + | == pBca1256-K112600 == | ||
| + | <pre> | ||
| + | Construction of <AP-tag> basic part K112600 | ||
| + | Wobble PCR SC001/SC002 (66 bp, EcoRI/BamHI) | ||
| + | Sub into pBca1256 (EcoRI/BamHI, 2227+ 910) | ||
| + | Product is pBca1256-K112600 | ||
| + | ---------------------------------------------------- | ||
| + | SC001F Forward Biobricking of <AP-tag> | ||
| + | cgataGAATTCatgAGATCTggcctgaacgatatttttgaagcgcag | ||
| + | SC002R Reverse Biobricking of <AP-tag> | ||
| + | ccagtGGATCCttcatgccattcaattttctgcgcttcaaaaatatcg | ||
| + | |||
| + | </pre> | ||
| + | |||
| + | |||
| + | == pBca1256-K112601 == | ||
| + | <pre> | ||
| + | Construction of <AP-tag! basic part K112601 | ||
| + | |||
| + | Wobble PCR SC001/SC003 (69 bp, EcoRI/BamHI) | ||
| + | Sub into pBca1256 (EcoRI/BamHI, 2227+ 910) | ||
| + | Product is pBca1256-K112601 | ||
| + | -------------------------------------------------- | ||
| + | SC001 Forward Biobricking of <AP-tag! | ||
| + | cgataGAATTCatgAGATCTggcctgaacgatatttttgaagcgcag | ||
| + | SC003 Reverse Biobricking of <AP-tag! | ||
| + | ccagtGGATCCttattcatgccattcaattttctgcgcttcaaaaatatcg | ||
Revision as of 18:49, 24 June 2008
pBca1256-K112600
Construction of <AP-tag> basic part K112600 Wobble PCR SC001/SC002 (66 bp, EcoRI/BamHI) Sub into pBca1256 (EcoRI/BamHI, 2227+ 910) Product is pBca1256-K112600 ---------------------------------------------------- SC001F Forward Biobricking of <AP-tag> cgataGAATTCatgAGATCTggcctgaacgatatttttgaagcgcag SC002R Reverse Biobricking of <AP-tag> ccagtGGATCCttcatgccattcaattttctgcgcttcaaaaatatcg
pBca1256-K112601
Construction of <AP-tag! basic part K112601 Wobble PCR SC001/SC003 (69 bp, EcoRI/BamHI) Sub into pBca1256 (EcoRI/BamHI, 2227+ 910) Product is pBca1256-K112601 -------------------------------------------------- SC001 Forward Biobricking of <AP-tag! cgataGAATTCatgAGATCTggcctgaacgatatttttgaagcgcag SC003 Reverse Biobricking of <AP-tag! ccagtGGATCCttattcatgccattcaattttctgcgcttcaaaaatatcg
