Wiki/Team:Warsaw/test notebook TOC
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- | <table class="month"><tr>< | + | <table class="month"><tr> |
+ | <tdalign=center><h2>Table of contents</h2></td><td> </td></tr> | ||
+ | <td width=70% valign=top style="border-left-style: grooved"> | ||
<h3><a onClick=switchMenu('one-armed-vect')> Preparation of one-armed bandit vectors</a></h3> | <h3><a onClick=switchMenu('one-armed-vect')> Preparation of one-armed bandit vectors</a></h3> | ||
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<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=5&arg0=21_July_2008&arg1=22_July_2008&arg2=23_July_2008&arg3=24_July_2008&arg4=25_July_2008&name=Cloning%20of%20protein%20A%20DNA%20to%20OmpA%20constructs>Cloning of protein A DNA to OmpA constructs </a></li> | <li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=5&arg0=21_July_2008&arg1=22_July_2008&arg2=23_July_2008&arg3=24_July_2008&arg4=25_July_2008&name=Cloning%20of%20protein%20A%20DNA%20to%20OmpA%20constructs>Cloning of protein A DNA to OmpA constructs </a></li> | ||
<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=3&arg0=29_July_2008&arg1=30_July_2008&arg2=31_July_2008&name=Cloning%20of%20omega_%26Delta%3BA%20DNA%20fragment%20to%20pACYC177%20OmpA_alpha>Cloning of omega_ΔA DNA fragment to pACYC177 OmpA_alpha </a></li> | <li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=3&arg0=29_July_2008&arg1=30_July_2008&arg2=31_July_2008&name=Cloning%20of%20omega_%26Delta%3BA%20DNA%20fragment%20to%20pACYC177%20OmpA_alpha>Cloning of omega_ΔA DNA fragment to pACYC177 OmpA_alpha </a></li> | ||
- | <li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=5&arg0=30_July_2008&arg1=31_July_2008&arg2=1_August_2008&arg3=4_August_2008&arg4=5_August_2008&name=Cloning%20of%20truncated%20fragment%20of%20protein%20A%20%28%26Delta%3BA%29>Cloning of truncated fragment of protein A ( | + | <li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=5&arg0=30_July_2008&arg1=31_July_2008&arg2=1_August_2008&arg3=4_August_2008&arg4=5_August_2008&name=Cloning%20of%20truncated%20fragment%20of%20protein%20A%20%28%26Delta%3BA%29>Cloning of truncated fragment of protein A (ΔA) </a></li> |
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<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=5&arg0=18_August_2008&arg1=19_August_2008&arg2=20_August_2008&arg3=21_August_2008&arg4=22_August_2008&name=Cloning%20of%20protein%20A%20DNA%20to%20GeneArt%20plasmid>Cloning of protein A DNA to GeneArt plasmid </a></li> | <li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=5&arg0=18_August_2008&arg1=19_August_2008&arg2=20_August_2008&arg3=21_August_2008&arg4=22_August_2008&name=Cloning%20of%20protein%20A%20DNA%20to%20GeneArt%20plasmid>Cloning of protein A DNA to GeneArt plasmid </a></li> | ||
- | <li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=4&arg0=4_August_2008&arg1=5_August_2008&arg2=6_August_2008&arg3=7_August_2008&name=Preparing%20pACYC177%20OmpA_omega_%26Delta%3BA%20construct">Preparing pACYC177 | + | <li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=4&arg0=4_August_2008&arg1=5_August_2008&arg2=6_August_2008&arg3=7_August_2008&name=Preparing%20pACYC177%20OmpA_omega_%26Delta%3BA%20construct">Preparing pACYC177 OmpA_omega_ΔA construct</a> |
</li> | </li> | ||
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<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=2&arg0=12_August_2008&arg1=13_August_2008&name=Checking%20for%20presence%20of%20protein%20A%20on%20the%20cell%20membrane>Checking for presence of protein A on the cell membrane </a></li> | <li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=2&arg0=12_August_2008&arg1=13_August_2008&name=Checking%20for%20presence%20of%20protein%20A%20on%20the%20cell%20membrane>Checking for presence of protein A on the cell membrane </a></li> | ||
- | <li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=3&arg0=4_August_2008&arg1=5_August_2008&arg2=6_August_2008&name=Checking%20if%20OmpA_omega_%26Delta%3BA_alpha%20gives%20ampicillin%20resistance">Checking if | + | <li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=3&arg0=4_August_2008&arg1=5_August_2008&arg2=6_August_2008&name=Checking%20if%20OmpA_omega_%26Delta%3BA_alpha%20gives%20ampicillin%20resistance">Checking if OmpA_omega_ΔA_alpha gives ampicillin resistance</a> |
</li> | </li> | ||
- | <li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=1&arg0=5_August_2008&name=Checking%20OmpA_omega_%26Delta%3BA_alpha%20expression">Checking | + | <li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=1&arg0=5_August_2008&name=Checking%20OmpA_omega_%26Delta%3BA_alpha%20expression">Checking OmpA_omega_ΔA_alpha expression</a><br> |
</li> | </li> | ||
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<li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=2&arg0=17_September_2008&arg1=18_September_2008&name=Optimisation%20of%20primers%20for%20preparation%20of%20parts">Optimisation of primers for preparation of parts</a></li> | <li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=2&arg0=17_September_2008&arg1=18_September_2008&name=Optimisation%20of%20primers%20for%20preparation%20of%20parts">Optimisation of primers for preparation of parts</a></li> | ||
- | <li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=7&arg0=18_September_2008&arg1=19_September_2008&arg2=22_September_2008&arg3=23_September_2008&arg4=24_September_2008&arg5=25_September_2008&arg6=26_September_2008&name=Preparation%20of%20%26Delta%3BA%20%28BBa_K103003%29">Preparation of | + | <li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=7&arg0=18_September_2008&arg1=19_September_2008&arg2=22_September_2008&arg3=23_September_2008&arg4=24_September_2008&arg5=25_September_2008&arg6=26_September_2008&name=Preparation%20of%20%26Delta%3BA%20%28BBa_K103003%29">Preparation of ΔA (BBa_K103003)</a></li> |
<li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=10&arg0=18_September_2008&arg1=2_October_2008&arg2=3_October_2008&arg3=6_October_2008&arg4=7_October_2008&arg5=8_October_2008&arg6=13_October_2008&arg7=14_October_2008&arg8=15_October_2008&arg9=16_October_2008&name=Preparation%20of%20linker_alpha%20%28BBa_K103009%29">Preparation of linker_alpha (BBa_K103009)</a></li> | <li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=10&arg0=18_September_2008&arg1=2_October_2008&arg2=3_October_2008&arg3=6_October_2008&arg4=7_October_2008&arg5=8_October_2008&arg6=13_October_2008&arg7=14_October_2008&arg8=15_October_2008&arg9=16_October_2008&name=Preparation%20of%20linker_alpha%20%28BBa_K103009%29">Preparation of linker_alpha (BBa_K103009)</a></li> | ||
<li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=6&arg0=18_September_2008&arg1=2_October_2008&arg2=3_October_2008&arg3=6_October_2008&arg4=7_October_2008&arg5=8_October_2008&name=Preparation%20of%20linker_omega%20%28BBa_K103013%29">Preparation of linker_omega (BBa_K103013)</a></li> | <li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=6&arg0=18_September_2008&arg1=2_October_2008&arg2=3_October_2008&arg3=6_October_2008&arg4=7_October_2008&arg5=8_October_2008&name=Preparation%20of%20linker_omega%20%28BBa_K103013%29">Preparation of linker_omega (BBa_K103013)</a></li> |
Revision as of 23:21, 28 October 2008
ProtocolsPrimersVectorsStrainsRosetta(DE3)pLysSF- ompT hsdSB(RB- mB-) gal dcm λ(DE3 [lacI lacUV5-T7 gene 1 ind1 sam7 nin5]) pLysSRARE (CamR) TOP10 (Invitrogen)F- mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 nupG recA1 araD139 Δ(ara-leu)7697 galE15 galK16 rpsL(StrR) endA1 λ- NR9458 (IBB collection; in notebook called mutD5)ara thi Δ pro lac mutD5 zaf-13 ::Tn10 GM2163ara-14 leuB6 fhuA31 lacY1 tsx78 glnV44 galK2 galT22 mcrA dcm-6 hisG4 rfbD1 R(zgb210::Tn10)TetS endA1 rpsL136 dam13::Tn9 xylA-5 mtl-1 thi-1 mcrB1 hsdR2 |