Wisconsin:Lignin Project/7 August 2008

From 2008.igem.org

(Difference between revisions)
 
Line 6: Line 6:
|'''Team Sorbitol:'''<br>
|'''Team Sorbitol:'''<br>
Set up a Digestion of the srlD PCR product as well as the pET28a vector.  The Products were verified on a gel and extracted using a gel purification kit.<br>
Set up a Digestion of the srlD PCR product as well as the pET28a vector.  The Products were verified on a gel and extracted using a gel purification kit.<br>
-
Performed practice assay on sheep liver soritol dehydrogenase.  A statistically significant amount of replicants were done for eventual E.coli SDH comparison assays.
+
Performed practice assay on sheep liver sorbitol dehydrogenase.  A statistically significant amount of replicants were done for eventual E.coli SDH comparison assays.
'''Team Fungus:'''<br>
'''Team Fungus:'''<br>

Latest revision as of 00:20, 29 October 2008

Igemwibanner.gif
Team Sorbitol:

Set up a Digestion of the srlD PCR product as well as the pET28a vector. The Products were verified on a gel and extracted using a gel purification kit.
Performed practice assay on sheep liver sorbitol dehydrogenase. A statistically significant amount of replicants were done for eventual E.coli SDH comparison assays.

Team Fungus:
Transformed into Rosetta-gami 2(DE3)pLysS.
Made overnight cultures of colonies 5 & 6 in LB + KAN (30 ug/mL) media.