Revision as of 00:42, 29 October 2008

Values in µM, min, and mL unless specified.

Parameter	Value	Source
Population and culture
k_n	3.85e-3	[1] observed division rate
N_max	1e8	[1]
V	1e-11	[1]
k_dil	varied	[1]
IP binding to receptor
k_assoc	5e-3	[1]
k_dissoc	4.55e-3	[1]
Synthesis of CRE1, YPD1, SKN7 species
k_s1	6.16e-5	[1]
k_s2	6.00e-4	[1]
k_s3	2.46e-4	[1]
k_dimer	20	[1]
Phosphorylation/Dephosphorylation reactions
k_p1	0.1	[1]
k_p2	1243	[1]
k_p3	56	[1]
k_p-3	4.8	[1]
k_dp1	5.33e-2	[1]
k_dp3	2.89e-3	[1]
k_dp3	4.80e-3	[1]
Decay constants
k_d1	5.33e-2	[1]
k_d2	2.89e-3	[1]
k_d3	4.80e-3	[1]
k_dip	5e-4	[1]
k_dgfp	5.77e-3	[1]
k_dtet	3.85e-3	Cell division rate (assumed stable)
k_dckx	<=3.85e-3	Cell division rate, or lower if diffusion is significant.
Basal expression
k_bgfp, k_btet, k_bckx	6e-6	adapted from [1]
k_tdim	1e3	arbitrary
Induced expression
k_gfp	6e-3	adapted from [1]
k_tet	varied	-
k_ckx	varied	-
K_g, K_t, K_c	varied	-
α_g, α_t α_c	varied	-
K_i	5e-6	K_ass = 2e11 M^-1 [2, 3]
β	varied	-
k_ipt4	varied	-
IPT4 enzyme kinetics		-
k_sip	Varied	-
CKX enzyme kinetics
K_m	40 µM	[ ]
k_cat	0.5-1000 s^-1	[ ], [ ]

References

Weiss, R. and M.-T. Chen (2005). "Artificial cell-cell communication in yeast Saccharomyces cerevisiae using signaling elements from Arabidopsis thaliana." Nature Biotechnology 23(12): 1551-1555.
Hillen, W. and C. Berens (1994). "Mechanisms underlying expression of Tn10 encoded tetracycline resistance." Annu. Rev. Microbiol. 48: 345-369.
Kleinschmidt, C., K. Tovar, et al. (1987). "Dynamics of repressor-operator recognition:Tn10 encoded tetracycline resistance control." Biochemistry 27: 1094-1104.
Schmulling T, Werner T, Riefler M, Krupkova E, Bartrina y Manns I (2003) Structure and function of cytokinin oxidase/dehydrogenase genes of maize, rice, Arabidopsis and other species. J Plant Res 116:241-252
Laskey, J.G., P. Patterson, et al. (2003). "Rate enhancement of cytokinin oxidase/dehydrogenase using 2,6-dichloroindophenol as an electron acceptor." Plant Growth Regulation 40: 189–196.
Frebortova, J., M.W. Fraaije, et al. (2004). "Catalytic reaction of cytokinin dehydrogenase: preference for quinones as electron acceptors." Biochem. J. 380: 121–130.

Blake, W., G. Balazsi, et al. (2006). "Phenotypic consequences of promoter-mediated transcriptional noise." Molecular Cell 24: 853-865.

@@ Line 170: / Line 170: @@
 # Weiss, R. and M.-T. Chen (2005). "Artificial cell-cell communication in yeast Saccharomyces cerevisiae using signaling elements from Arabidopsis thaliana." Nature Biotechnology 23(12): 1551-1555.
 # Hillen, W. and C. Berens (1994). "Mechanisms underlying expression of Tn10 encoded tetracycline resistance." Annu. Rev. Microbiol. 48: 345-369.
+#Kleinschmidt, C., K. Tovar, et al. (1987). "Dynamics of repressor-operator recognition:Tn10 encoded tetracycline resistance control." Biochemistry 27: 1094-1104.
-Kleinschmidt, C., K. Tovar, et al. (1987). "Dynamics of repressor-operator recognition:Tn10 encoded tetracycline resistance control." Biochemistry 27: 1094-1104.
 # Schmulling T, Werner T, Riefler M, Krupkova E, Bartrina y Manns I (2003) Structure and function of cytokinin oxidase/dehydrogenase genes of maize, rice, Arabidopsis and other species. J Plant Res 116:241-252
 # Laskey, J.G., P. Patterson, et al. (2003). "Rate enhancement of cytokinin oxidase/dehydrogenase using 2,6-dichloroindophenol as an electron acceptor." Plant Growth Regulation 40: 189–196.
 # Frebortova, J., M.W. Fraaije, et al. (2004). "Catalytic reaction of cytokinin dehydrogenase: preference for quinones as electron acceptors." Biochem. J. 380: 121–130.