Wiki/Team:Warsaw/igem notebook.htm
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<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=2&arg0=14_July_2008&arg1=15_July_2008&name=Preparation%20of%20alpha%20A%20conctruct>Preparation of alpha A conctruct </a></li> | <li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=2&arg0=14_July_2008&arg1=15_July_2008&name=Preparation%20of%20alpha%20A%20conctruct>Preparation of alpha A conctruct </a></li> | ||
<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=5&arg0=21_July_2008&arg1=22_July_2008&arg2=23_July_2008&arg3=24_July_2008&arg4=25_July_2008&name=Cloning%20of%20protein%20A%20DNA%20to%20OmpA%20constructs>Cloning of protein A DNA to OmpA constructs </a></li> | <li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=5&arg0=21_July_2008&arg1=22_July_2008&arg2=23_July_2008&arg3=24_July_2008&arg4=25_July_2008&name=Cloning%20of%20protein%20A%20DNA%20to%20OmpA%20constructs>Cloning of protein A DNA to OmpA constructs </a></li> | ||
- | <li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=3&arg0=29_July_2008&arg1=30_July_2008&arg2=31_July_2008&name=Cloning%20of%20omega_%26Delta%3BA%20DNA%20fragment%20to%20pACYC177%20OmpA_alpha>Cloning of | + | <li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=3&arg0=29_July_2008&arg1=30_July_2008&arg2=31_July_2008&name=Cloning%20of%20omega_%26Delta%3BA%20DNA%20fragment%20to%20pACYC177%20OmpA_alpha>Cloning of omega_ΔA DNA fragment to pACYC177 OmpA_alpha </a></li> |
<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=5&arg0=30_July_2008&arg1=31_July_2008&arg2=1_August_2008&arg3=4_August_2008&arg4=5_August_2008&name=Cloning%20of%20truncated%20fragment%20of%20protein%20A%20%28%26Delta%3BA%29>Cloning of truncated fragment of protein A (ΔA) </a></li> | <li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=5&arg0=30_July_2008&arg1=31_July_2008&arg2=1_August_2008&arg3=4_August_2008&arg4=5_August_2008&name=Cloning%20of%20truncated%20fragment%20of%20protein%20A%20%28%26Delta%3BA%29>Cloning of truncated fragment of protein A (ΔA) </a></li> | ||
Revision as of 01:24, 29 October 2008
ProtocolsPrimersVectorsStrainsRosetta(DE3)pLysSF- ompT hsdSB(RB- mB-) gal dcm λ(DE3 [lacI lacUV5-T7 gene 1 ind1 sam7 nin5]) pLysSRARE (CamR) TOP10 (Invitrogen)F- mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 nupG recA1 araD139 Δ(ara-leu)7697 galE15 galK16 rpsL(StrR) endA1 λ- NR9458 (IBB collection; in notebook called mutD5)ara thi Δ pro lac mutD5 zaf-13 ::Tn10 GM2163ara-14 leuB6 fhuA31 lacY1 tsx78 glnV44 galK2 galT22 mcrA dcm-6 hisG4 rfbD1 R(zgb210::Tn10)TetS endA1 rpsL136 dam13::Tn9 xylA-5 mtl-1 thi-1 mcrB1 hsdR2 |