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Restriction Map/ Restriction Digest
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Revision as of 02:25, 29 October 2008
Restriction Map Design
Restriction maps are designed through Vector NTI plasmid sequences. Restriction sites with compatible enzymes (similar temperatures and buffers used) are found, and those enzymes are then used to digest the vector plasmid. The restriction site must also be in the proper area of the plasmid for insertion of other necessary isolated DNA. The inserted DNA must also have the cut sites, so areas of DNA will overlap when the plasmid is ligated.
Restriction Digest Protocol
Calculations
