"
Team:UCSF/Parts
From 2008.igem.org
| Line 45: | Line 45: | ||
'''AarI Shuttle Vector''' | '''AarI Shuttle Vector''' | ||
| - | |||
''So you don't want to use AarI cloning? OK. We've provided a shuttle vector allows one-sublcone conversion of AarI parts into biobricks. | ''So you don't want to use AarI cloning? OK. We've provided a shuttle vector allows one-sublcone conversion of AarI parts into biobricks. | ||
Revision as of 04:43, 29 October 2008
For complete details and sequence, see the Registry: http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2008&group=UCSF&Done=1
Project-relevant AarI TOPO clones
digestion of these plasmids with AarI will release a fragment for AarI cloning.
- LexA (1-87 a.a) AB
- Sir2 BD
- GFP AD
- Ssn8 AD
- Sir3 A!D
- Sir4 AD
GFP BD
Sir4
Sir2 AB
mCherry AB
mCherry BD
LexA (1-87) BD
Pif3 AB
PhyB AB
Sas2 AB
Sas2 BD
AarI acceptor vectors (empty) These accept AarI digested parts, and include a promoter/terminator.
AarI Shuttle Vector So you don't want to use AarI cloning? OK. We've provided a shuttle vector allows one-sublcone conversion of AarI parts into biobricks.
- AarI--Biobrick Shuttle AB
- AarI--Biobrick Shuttle BD
Bonus AarI Parts
These are provided to the registry to facilitate getting started with AarI cloning. Some we had planned to use, but didn't get around to, and others are selected from the lab database of AarI parts.
Esa1 AB
| Home | The Team | The Project | Parts Submitted to the Registry | Modeling | Human Practices | Notebooks |
|---|
