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Team:Bologna/Wetlab
From 2008.igem.org
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| + | =LAB Experiment = | ||
| + | In our genetic bi-stable, the amount of molecules produced to switch the system from the two possible steady state is ruled by LacI and TetR protein. The production of LacI molecules by UV induction can be tested replacing the LacI gene by GFP, so it is possible to have a relation of the strength of LacI synthesis measuring the value of its fluorescence. | ||
| + | BBa_K079049 and BBa_K079050 are two new construct submitted to the registry by Bologna’s Igem Team 2008. These UV test circuits can be presented schematically by the following sequence: | ||
| + | [[Image:minicirc2.jpg|center]] | ||
| + | * Promoter Constitutive Family: BBa_J23118 (1429 strength ) or BBa_23100 (2547 strength); | ||
| + | * LexA Operator Site Binding (K079040 ) ; | ||
| + | * Rbs with Green fluorescence Protein LVA and Terminator (BBa_I763020) | ||
| + | <br> | ||
| + | In order to generate the induction by Uv, a box with UV lamp was realized. | ||
= Single operator isolation = | = Single operator isolation = | ||
Revision as of 09:30, 29 October 2008
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LAB Experiment
In our genetic bi-stable, the amount of molecules produced to switch the system from the two possible steady state is ruled by LacI and TetR protein. The production of LacI molecules by UV induction can be tested replacing the LacI gene by GFP, so it is possible to have a relation of the strength of LacI synthesis measuring the value of its fluorescence. BBa_K079049 and BBa_K079050 are two new construct submitted to the registry by Bologna’s Igem Team 2008. These UV test circuits can be presented schematically by the following sequence:
- Promoter Constitutive Family: BBa_J23118 (1429 strength ) or BBa_23100 (2547 strength);
- LexA Operator Site Binding (K079040 ) ;
- Rbs with Green fluorescence Protein LVA and Terminator (BBa_I763020)
In order to generate the induction by Uv, a box with UV lamp was realized.
