Wiki/Team:Warsaw/igem notebook.htm
From 2008.igem.org
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<li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=2&arg0=24_September_2008&arg1=25_September_2008&name=Preparation%20of%20alpha_A%20construct">Preparation of alpha_A construct</a></li> | <li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=2&arg0=24_September_2008&arg1=25_September_2008&name=Preparation%20of%20alpha_A%20construct">Preparation of alpha_A construct</a></li> | ||
- | <li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=4&arg0=4_August_2008&arg1=5_August_2008&arg2=6_August_2008&arg3=7_August_2008&name=Preparing%20pACYC177%20OmpA_omega_%26Delta%3BA%20construct>Preparing pACYC177 | + | <li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=4&arg0=4_August_2008&arg1=5_August_2008&arg2=6_August_2008&arg3=7_August_2008&name=Preparing%20pACYC177%20OmpA_omega_%26Delta%3BA%20construct>Preparing pACYC177 OmpA_omega_ΔA construct </a></li> |
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- | <li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=9&arg0=13_August_2008&arg1=14_August_2008&arg2=18_August_2008&arg3=19_August_2008&arg4=20_August_2008&arg5=21_August_2008&arg6=22_August_2008&arg7=25_August_2008&arg8=26_August_2008&name=Cloning%20of%20alpha%20to%20pACYC177%20OmpA_A_omega%20and%20pACYC177%20OmpA_Z_omega>Cloning of alpha to pACYC177 OmpA_A_omega and pACYC177 OmpA_Z_omega </a></li> | + | <li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=9&arg0=13_August_2008&arg1=14_August_2008&arg2=18_August_2008&arg3=19_August_2008&arg4=20_August_2008&arg5=21_August_2008&arg6=22_August_2008&arg7=25_August_2008&arg8=26_August_2008&name=Cloning%20of%20alpha%20to%20pACYC177%20OmpA_A_omega%20and%20pACYC177%20OmpA_Z_omega">Cloning of alpha to pACYC177 OmpA_A_omega and pACYC177 OmpA_Z_omega</a></li> |
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<div name="submenu_item" id="expr-tests" style="display: none"> | <div name="submenu_item" id="expr-tests" style="display: none"> | ||
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- | <li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=1&arg0=4_August_2008&name=Checking%20OmpA_omega_%26Delta%3BA_alpha%20and%20OmpA_A_alpha%20expression>Checking | + | <li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=1&arg0=4_August_2008&name=Checking%20OmpA_omega_%26Delta%3BA_alpha%20and%20OmpA_A_alpha%20expression>Checking OmpA_omega_ΔA_alpha and OmpA_A_alpha expression </a></li> |
- | <li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=1&arg0=5_August_2008&name=Checking%20OmpA_omega_%26Delta%3BA_alpha%20expression>Checking | + | <li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=1&arg0=5_August_2008&name=Checking%20OmpA_omega_%26Delta%3BA_alpha%20expression>Checking OmpA_omega_ΔA_alpha expression </a></li> |
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<ul> | <ul> | ||
- | <li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=3&arg0=4_August_2008&arg1=5_August_2008&arg2=6_August_2008&name=Checking%20if%20OmpA_omega_%26Delta%3BA_alpha%20gives%20ampicillin%20resistance>Checking if | + | <li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=3&arg0=4_August_2008&arg1=5_August_2008&arg2=6_August_2008&name=Checking%20if%20OmpA_omega_%26Delta%3BA_alpha%20gives%20ampicillin%20resistance>Checking if OmpA_omega_ΔA_alpha gives ampicillin resistance </a></li> |
<li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=10&arg0=19_August_2008&arg1=20_August_2008&arg2=21_August_2008&arg3=22_August_2008&arg4=25_August_2008&arg5=26_August_2008&arg6=27_August_2008&arg7=9_September_2008&arg8=10_September_2008&arg9=11_September_2008&name=Tests%20for%20ampicillin%20resistance%20given%20by%20protein%20added%20to%20medium">Tests for ampicillin resistance given by protein added to medium</a></li> | <li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=10&arg0=19_August_2008&arg1=20_August_2008&arg2=21_August_2008&arg3=22_August_2008&arg4=25_August_2008&arg5=26_August_2008&arg6=27_August_2008&arg7=9_September_2008&arg8=10_September_2008&arg9=11_September_2008&name=Tests%20for%20ampicillin%20resistance%20given%20by%20protein%20added%20to%20medium">Tests for ampicillin resistance given by protein added to medium</a></li> | ||
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<h2 align="left">Strains</h2> | <h2 align="left">Strains</h2> | ||
<h4><a name="rosetta">Rosetta(DE3)pLysS</a></h4> | <h4><a name="rosetta">Rosetta(DE3)pLysS</a></h4> | ||
- | <p>F- ompT hsdSB(RB- mB-) gal dcm | + | <p>F- ompT hsdSB(RB- mB-) gal dcm λ(DE3 [lacI lacUV5-T7 gene 1 ind1 sam7 nin5]) pLysSRARE (CamR)</p> |
<h4><a name="top10">TOP10 (Invitrogen)</a></h4> | <h4><a name="top10">TOP10 (Invitrogen)</a></h4> | ||
- | <p>F- mcrA | + | <p>F- mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 nupG recA1 araD139 Δ(ara-leu)7697 galE15 galK16 rpsL(StrR) endA1 λ- </p> |
<h4><a name="mutD5">NR9458 (IBB collection; in notebook called mutD5)</a></h4> | <h4><a name="mutD5">NR9458 (IBB collection; in notebook called mutD5)</a></h4> | ||
- | <p>ara thi | + | <p>ara thi Δ pro lac mutD5 zaf-13 ::Tn10</p> |
<h4><a name="gm">GM2163</a></h4> | <h4><a name="gm">GM2163</a></h4> | ||
<p>ara-14 leuB6 fhuA31 lacY1 tsx78 glnV44 galK2 galT22 mcrA dcm-6 hisG4 rfbD1 | <p>ara-14 leuB6 fhuA31 lacY1 tsx78 glnV44 galK2 galT22 mcrA dcm-6 hisG4 rfbD1 |
Revision as of 11:14, 29 October 2008
ProtocolsPrimersVectorsJavaScript tools developed to make working with iGEM notebook more comfortableStrainsRosetta(DE3)pLysSF- ompT hsdSB(RB- mB-) gal dcm λ(DE3 [lacI lacUV5-T7 gene 1 ind1 sam7 nin5]) pLysSRARE (CamR) TOP10 (Invitrogen)F- mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 nupG recA1 araD139 Δ(ara-leu)7697 galE15 galK16 rpsL(StrR) endA1 λ- NR9458 (IBB collection; in notebook called mutD5)ara thi Δ pro lac mutD5 zaf-13 ::Tn10 GM2163ara-14 leuB6 fhuA31 lacY1 tsx78 glnV44 galK2 galT22 mcrA dcm-6 hisG4 rfbD1 R(zgb210::Tn10)TetS endA1 rpsL136 dam13::Tn9 xylA-5 mtl-1 thi-1 mcrB1 hsdR2 |