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Revision as of 10:01, 29 October 2008
A Genetic Limiter Circuit in S. cerevisiae
We have designed a modular genetic circuit to limit the expression level of a gene of interest to a user-defined, tunable threshold. The limiter network reacts to the transcription of an endogenous gene within each cell, entering a regulatory state only where and when the rate of transcription lies beyond an acceptable range of activity. Along with its potential therapeutic utility, we offer our device as a foundational tool for researching gene expression in a eukaryotic model.
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