Team:Chiba/Sender experiments/Senders(XL10Gold) T9002(JW1908)

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Revision as of 11:12, 29 October 2008

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design

Sender

[http://partsregistry.org/Part:BBa_K084007 plac+rbs+LasI(BBa_K084007)]

[http://partsregistry.org/Part:BBa_K084008 plac+rbs+RhlI(BBa_K084008)]

[http://partsregistry.org/Part:BBa_K084009 BBa_K084009]
[http://partsregistry.org/Part:BBa_K084012 BBa_K084012]

[http://partsregistry.org/Part:BBa_K084014 BBa_K084014]

[http://partsregistry.org/Part:BBa_S03623 BBa_S03623(ptet+rbs+LuxI(LVA))]

Receiver

[http://partsregistry.org/Part:BBa_T9002 BBa_T9002 (Express GFP in response to AHL)]

Method

Transformed Senders into E.coli strains(JW1908) and Receiver into E.coli strain(JW1908).
Inoculated them independently in liquid media. Incubated at 37°C 12h
Mixed them.
Incubated at 37°C.
Measured intensity of green fluorescence at regular time intervals.

more details...

Results

Reaction temparature:37°C

センダーの培養液：500μL、レシーバの培養液：500μL

Fig.　　
E.coli strain,BBa_K084007:XL10Gold,BBa_T9002:JW1908,37°C,Receiver cells/Sender cells = 1.

Fig.　　
E.coli strain,BBa_K084007:XL10Gold,BBa_T9002:JW1908,37°C,Receiver cells/Sender cells = 1.

Fig.　　
E.coli strain,BBa_K084007:XL10Gold,BBa_T9002:JW1908,37°C,Receiver cells/Sender cells = 1.

Reaction temparature:30°C

センダーの培養液：500μL、レシーバの培養液：500μL

Fig.　　
E.coli strain,BBa_K084007:XL10Gold,BBa_T9002:JW1908,30°C,Receiver cells/Sender cells = 1.

Fig.　　
E.coli strain,BBa_K084007:XL10Gold,BBa_T9002:JW1908,30°C,Receiver cells/Sender cells = 1.

Reaction temparature:25°C

Back to Sender experiment and result

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