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Team:Rice University/Notebook/4 June 2008
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***lambda DNA with HindIII only | ***lambda DNA with HindIII only | ||
*Taylor Stevenson | *Taylor Stevenson | ||
| - | **Titered the phage stocks obtained from packaging the NEB lambda genome. See | + | **Titered the phage stocks obtained from packaging the NEB lambda genome. See packaging manual [https://static.igem.org/mediawiki/2008/6/6a/Packaging_Extract.pdf]. |
**'''Result'''-no colonies grew with a 1/200 (phage mixture:cell culture) was plated. Additionally, the more diluted phage mixture showed a titer of approximately 10^5 pfu/uL. | **'''Result'''-no colonies grew with a 1/200 (phage mixture:cell culture) was plated. Additionally, the more diluted phage mixture showed a titer of approximately 10^5 pfu/uL. | ||
<BR<BR><BR><BR> | <BR<BR><BR><BR> | ||
Latest revision as of 20:40, 16 July 2008
Wednesday 4 June
- Selim Sheikh:
- Prepared 2 digestion solutions:
- lambda DNA with XhoI and HindIII
- lambda DNA with HindIII only
- Prepared 2 digestion solutions:
- Taylor Stevenson
- Titered the phage stocks obtained from packaging the NEB lambda genome. See packaging manual [1].
- Result-no colonies grew with a 1/200 (phage mixture:cell culture) was plated. Additionally, the more diluted phage mixture showed a titer of approximately 10^5 pfu/uL.
<BR
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