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Purdue/25 June 2008
From 2008.igem.org
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Utilized UV Vis in Room 234 to get an absorbance reading from DNA plasmid to determine the amount of DNA on a single punch from the part kit. | Utilized UV Vis in Room 234 to get an absorbance reading from DNA plasmid to determine the amount of DNA on a single punch from the part kit. | ||
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| + | Analyzation revealed approximately 112 ug/mL, which translates to 1 ug per spot (or punch). The second transformation above used two spots of DNA. | ||
Revision as of 19:16, 25 June 2008
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Second Attempt at Transformations
Obtained a new set of Chemically Compotent Cells from Larisa. Cells are considered Max Efficiency.
UV Vis Spectroscopy Data from Plasmid
Utilized UV Vis in Room 234 to get an absorbance reading from DNA plasmid to determine the amount of DNA on a single punch from the part kit.
Analyzation revealed approximately 112 ug/mL, which translates to 1 ug per spot (or punch). The second transformation above used two spots of DNA.
