Team:PennState/diauxie/progress

We have completed our first set of cloning phases and are conducting our first round of testing and modification. Each test construct (promoter + GFP) has been cloned into pSB1A2 and transformed into DH5α, W3110 ∆xylB-G, and W3110 ∆xylB-R. Preliminary induction studies were run to find the optimal induction time and to see in what range OD and fluorescence were linearly related.

Test Construct

Test were then run to compare the level of induction between mixes of xylose, glucose and xylose, and glucose. The goal of this project is to obtain a noticeably higher level of induction when induced with xylose and glucose compared to the wildtype construct.

DH5a fluoresence

W3110 fluoresence

PN induction time

P1 induction time

P3 induction time

PN linear range

P1 linear range

P3 linear range

@@ Line 229: / Line 229: @@
 <div id="psugallery" style="float: right; clear: right; width: 500px;">
   <div class="gallerybox" style="width: 155px; float: left; clear: none;">
-   <div class="thumb" style="padding: 18px 0pt; width: 150px;"><div style="margin: auto; width: 120px;"><a href="/Image:DH5a_fluor_penn_state08.jpg" class="image" title="Team member 1.png"><img alt="" src="/wiki/images/0/0f/DH5a_fluor_penn_state08.jpg" border="0" height="110" width="120"></a></div></div>
+   <div class="thumb" style="padding: 18px 0pt; width: 150px;"><div style="margin: auto; width: 120px;"><a href="/Image:DH5a_fluor_penn_state08.jpg" class="image" title="DH5a_fluor_penn_state08.jpg"><img alt="" src="/wiki/images/0/0f/DH5a_fluor_penn_state08.jpg" border="0" height="110" width="120"></a></div></div>
    <div class="gallerytext"><p>DH5a fluoresence</p></div>
   </div>
@@ Line 236: / Line 236: @@
 <div id="psugallery" style="float: right; clear: right; width: 500px;">
   <div class="gallerybox" style="width: 155px; float: left; clear: none;">
-   <div class="thumb" style="padding: 18px 0pt; width: 150px;"><div style="margin: auto; width: 120px;"><a href="/Image:W3110_fluor_penn_state08.jpg" class="image" title="Team member 1.png"><img alt="" src="/wiki/images/8/83/W3110_fluor_penn_state08.jpg" border="0" height="110" width="120"></a></div></div>
+   <div class="thumb" style="padding: 18px 0pt; width: 150px;"><div style="margin: auto; width: 120px;"><a href="/Image:W3110_fluor_penn_state08.jpg" class="image" title="W3110_fluor_penn_state08.jpg"><img alt="" src="/wiki/images/8/83/W3110_fluor_penn_state08.jpg" border="0" height="110" width="120"></a></div></div>
    <div class="gallerytext"><p>W3110 fluoresence</p></div>
   </div>
@@ Line 250: / Line 250: @@
 <div id="psugallery" style="float: right; clear: right; width: 500px;">
   <div class="gallerybox" style="width: 155px; float: left; clear: none;">
-   <div class="thumb" style="padding: 18px 0pt; width: 150px;"><div style="margin: auto; width: 120px;"><a href="/Image:P1_time_penn_state08.jpg" class="image" title="Team member 1.png"><img alt="" src="/wiki/images/8/8d/P1_time_penn_state08.jpg" border="0" height="110" width="120"></a></div></div>
+   <div class="thumb" style="padding: 18px 0pt; width: 150px;"><div style="margin: auto; width: 120px;"><a href="/Image:P1_time_penn_state08.jpg" class="image" title="P1_time_penn_state08.jpg"><img alt="" src="/wiki/images/8/8d/P1_time_penn_state08.jpg" border="0" height="110" width="120"></a></div></div>
    <div class="gallerytext"><p>P1 induction time</p></div>
   </div>
@@ Line 271: / Line 271: @@
 <div id="psugallery" style="float: right; clear: right; width: 500px;">
   <div class="gallerybox" style="width: 155px; float: left; clear: none;">
-   <div class="thumb" style="padding: 18px 0pt; width: 150px;"><div style="margin: auto; width: 120px;"><a href="/Image:P1_linear_penn_state08.jpg" class="image" title="Team member 1.png"><img alt="" src="/wiki/images/c/c9/P1_linear_penn_state08.jpg" border="0" height="110" width="120"></a></div></div>
+   <div class="thumb" style="padding: 18px 0pt; width: 150px;"><div style="margin: auto; width: 120px;"><a href="/Image:P1_linear_penn_state08.jpg" class="image" title="P1_linear_penn_state08.jpg"><img alt="" src="/wiki/images/c/c9/P1_linear_penn_state08.jpg" border="0" height="110" width="120"></a></div></div>
    <div class="gallerytext"><p>P1 linear range</p></div>
   </div>
@@ Line 277: / Line 277: @@
 </div>
 <div id="psugallery" style="float: right; clear: right; width: 500px;">
-  <div class="gallerybox" style="width: 155px; float: left; clear: none;">
+  <div class="gallerybox" style="width: 205px; float: left; clear: none;">
-   <div class="thumb" style="padding: 18px 0pt; width: 150px;"><div style="margin: auto; width: 120px;"><a href="/Image:P3_linear_penn_state08.jpg" class="image" title="P3_linear_penn_state08.jpg"><img alt="" src="/wiki/images/f/f5/P3_linear_penn_state08.jpg" border="0" height="110" width="120"></a></div></div>
+   <div class="thumb" style="padding: 18px 0pt; width: 200px;"><div style="margin: auto; width: 120px;"><a href="/Image:P3_linear_penn_state08.jpg" class="image" title="P3_linear_penn_state08.jpg"><img alt="" src="/wiki/images/f/f5/P3_linear_penn_state08.jpg" border="0" height="110" width="120"></a></div></div>
    <div class="gallerytext"><p>P3 linear range</p></div>
   </div>