"
Lux signaling system
From 2008.igem.org
(→Lux cell signaling system) |
|||
| Line 3: | Line 3: | ||
'''Responsible:''' Andrew Gordon and Pallavi Penumetcha | '''Responsible:''' Andrew Gordon and Pallavi Penumetcha | ||
| - | '''Signal molecule:''' ''N''-acyl-homoserine lactone (AHL) Generic term for a variety of species specific hormone-like molecules | + | '''Signal molecule:''' ''N''-acyl-homoserine lactone (AHL) Generic term for a variety of species specific hormone-like molecules. The molecule of focus: 3-oxohexanoyl-homoserine lactone. |
'''Bacterial species:''' discovered in ''Vibrio fischeri'' known to work in ''E. coli'' | '''Bacterial species:''' discovered in ''Vibrio fischeri'' known to work in ''E. coli'' | ||
Revision as of 02:01, 30 October 2008
Lux cell signaling system
Responsible: Andrew Gordon and Pallavi Penumetcha
Signal molecule: N-acyl-homoserine lactone (AHL) Generic term for a variety of species specific hormone-like molecules. The molecule of focus: 3-oxohexanoyl-homoserine lactone.
Bacterial species: discovered in Vibrio fischeri known to work in E. coli
Receiver protein: LuxR protein receives signal from AHL; also has some control over transciption of luciferase
Signal molecule synthase: LuxI; also has some control over transciption of luciferase
Additional Information: "Quorum Quenching" aiiA (intracellular) lactonase reduces AHL concentration
Resources
[http://partsregistry.org/Lux Lux Operon Pathway]
[http://partsregistry.org/AHL AHL signaling molecules by species; some are specific to gram pos but may affect gram negs]
[http://www.horizonpress.com/jmmb/v1/v1n1/03.pdf Reducing Crosstalk in Lux System]
References
[http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=522112 Quorum Quenching to control Lux Pathway]
[http://www.nature.com/nature/journal/v434/n7037/full/nature03461.html] A Synthetic multicellular system for programmed pattern formation
