Wiki/Team:Warsaw/igem notebook.htm
From 2008.igem.org
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+ | <hr width="50%"> | ||
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+ | <h3 align="left"><a OnClick="switchMenu('protocols','submenu_item')">Protocols</a></h3> | ||
+ | <div name="submenu_item" id="protocols" style="display:none"> | ||
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+ | <ol> | ||
+ | <li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#Z_a_Z_o">Purification of His_Z_alpha and His_Z_omega</a></li> | ||
+ | <li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#A_a">Purification of His_A_alpha</a></li> | ||
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+ | <li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#Testing various hunter/prey combinations">Testing various hunter/prey combinations</a></li> | ||
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+ | <li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Plasmid DNA isolation</a></li> | ||
+ | <li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">DNA isolation from agarose gel</a></li> | ||
+ | <li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_purification_after_enzymatic_reaction">DNA purification after enzymatic reaction</a></li> | ||
+ | <li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#genomic_DNA_isolation">Genomic DNA isolation</a></li> | ||
+ | <li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#chemocompetent">Preparation of chemocompetent bacteria</a></li> | ||
+ | <li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#electrocompetent">Preparation of electrocompetent bacteria</a></li> | ||
+ | <li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#chemotransform">Chemotransformation</a></li> | ||
+ | <li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#electrotransform">Electrotransformation</a></li> | ||
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+ | <li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">Ligation</a></li> | ||
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+ | <li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#blunting">Blunting</a></li> | ||
+ | <li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#concentrations">Standard concentrations of antibiotics and other supplements | ||
+ | </a></li> | ||
+ | <li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#rif">Rifampicin test | ||
+ | </a></li> | ||
+ | <li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#removing">Removing 5' phosphate groups from DNA ends | ||
+ | </a></li> | ||
+ | <li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#taxi">TAXI protocol (Tet+Ap 30+X-Gal+IPTG) | ||
+ | </a></li> | ||
+ | <li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#bca">Protein concentration measurement (BCA method) | ||
+ | </a></li> | ||
+ | <li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">DNA digest | ||
+ | </a></li> | ||
+ | <li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR | ||
+ | </a></li> | ||
+ | <li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcl">Polymerase Chain Ligation (PCL) | ||
+ | </a></li> | ||
+ | |||
+ | </ol> | ||
+ | </div> | ||
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+ | <h3 align="left"><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/primers">Primers</a></h3> | ||
+ | <h3 align="left"><a href="https://2008.igem.org/Wiki/Team:Warsaw/vectors">Vectors</a></h3> | ||
+ | <h3 align="left"><a href="https://2008.igem.org/Wiki/Team:Warsaw/scripts">JavaScript tools developed to make work with iGEM notebook more comfortable</a></h3> | ||
+ | <h3 align="left">Strains</h3> | ||
+ | <h4><a name="rosetta">Rosetta(DE3)pLysS</a></h4> | ||
+ | <p>F- ompT hsdSB(RB- mB-) gal dcm λ(DE3 [lacI lacUV5-T7 gene 1 ind1 sam7 nin5]) pLysSRARE (CamR)</p> | ||
+ | <h4><a name="top10">TOP10 (Invitrogen)</a></h4> | ||
+ | <p>F- mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 nupG recA1 araD139 Δ(ara-leu)7697 galE15 galK16 rpsL(StrR) endA1 λ- </p> | ||
+ | <h4><a name="mutD5">NR9458 (IBB collection; in notebook called mutD5)</a></h4> | ||
+ | <p>ara thi Δ pro lac mutD5 zaf-13 ::Tn10</p> | ||
+ | <h4><a name="gm">GM2163</a></h4> | ||
+ | <p>ara-14 leuB6 fhuA31 lacY1 tsx78 glnV44 galK2 galT22 mcrA dcm-6 hisG4 rfbD1 | ||
+ | R(zgb210::Tn10)TetS endA1 rpsL136 dam13::Tn9 xylA-5 mtl-1 thi-1 mcrB1 | ||
+ | hsdR2</p> | ||
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Revision as of 15:30, 29 October 2008
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