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IGEM:Cambridge/2008/Notebook/Voltage/Output
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=Setup= | =Setup= | ||
Apparatus is setup as shown. An earth is needed next to the probe electrode, but not touching. They will be needed within the same solution to reduce the noise. | Apparatus is setup as shown. An earth is needed next to the probe electrode, but not touching. They will be needed within the same solution to reduce the noise. | ||
| - | *Cells containing biobrick K090002 are grown overnight in [[Team:Cambridge/Protocols#NOK_2.0_.28No_potassium_nutrient_broth.29| NOK]] with 5%(by vol.) 2M KCl added. | + | *Cells containing biobrick K090002 are grown overnight in [[Team:Cambridge/Protocols#NOK_2.0_.28No_potassium_nutrient_broth.29| NOK]] with 5%(by vol.) 2M KCl added - final 100mM K+. |
*Samples are then spun down in individual 1.5ml eppendorfs. | *Samples are then spun down in individual 1.5ml eppendorfs. | ||
Revision as of 16:57, 29 October 2008
Setup
Apparatus is setup as shown. An earth is needed next to the probe electrode, but not touching. They will be needed within the same solution to reduce the noise.
- Cells containing biobrick K090002 are grown overnight in NOK with 5%(by vol.) 2M KCl added - final 100mM K+.
- Samples are then spun down in individual 1.5ml eppendorfs.
Experiment
- Resuspend cells in 200μL clean NOK and place in sample wells
- Lower probe electrode into cell solution and wait for stable reading - 1min.
- Add sample solution
Results
