Team:University of Ottawa/24 June 2008

From 2008.igem.org

(Difference between revisions)
(New page: __TOC__ ==Today in the Lab== '''Matt''' :'''Genomic DNA extraction''' ::<li> Mila showed me how to do a genomic DNA extraction of the four YPH500 colonies. ::<li> Absorbance of colony A wh...)
(Today in the Lab)
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:'''Genomic DNA extraction'''
:'''Genomic DNA extraction'''
::<li> Mila showed me how to do a genomic DNA extraction of the four YPH500 colonies.
::<li> Mila showed me how to do a genomic DNA extraction of the four YPH500 colonies.
-
::<li> Absorbance of colony A when measuring DNA concentration was found to be 0.6641 - that colony was then chosen for PCR amplification of the PTP2 cassette.
+
::<li> Genomic DNA concentration of colony A was found to be 830.125 ng/ul  - that colony was then chosen for PCR amplification of the PTP2 cassette.
::<li> The PCR amplification will be performed tomorrow morning.
::<li> The PCR amplification will be performed tomorrow morning.

Revision as of 13:42, 26 June 2008

Contents

Today in the Lab

Matt

Genomic DNA extraction
  • Mila showed me how to do a genomic DNA extraction of the four YPH500 colonies.
  • Genomic DNA concentration of colony A was found to be 830.125 ng/ul - that colony was then chosen for PCR amplification of the PTP2 cassette.
  • The PCR amplification will be performed tomorrow morning.