"
Team:Paris/Quorum sensing
From 2008.igem.org
(→The genetic cascade) |
(→The genetic cascade) |
||
| Line 4: | Line 4: | ||
The phenomenon of ''quorum sensing'' was first discovered in ''Vibrio fischeri''. This bacteria only expresses luciferase when the density of bacteria is very high (10<sup>11</sup>bact/mL). Typically, the bacteria does not express bioluminescence when they are free living in the ocean. At the contrary, they express luciferase when they are highly concentrated in the photophore of Hawaiian bobtail squid. | The phenomenon of ''quorum sensing'' was first discovered in ''Vibrio fischeri''. This bacteria only expresses luciferase when the density of bacteria is very high (10<sup>11</sup>bact/mL). Typically, the bacteria does not express bioluminescence when they are free living in the ocean. At the contrary, they express luciferase when they are highly concentrated in the photophore of Hawaiian bobtail squid. | ||
===The genetic cascade=== | ===The genetic cascade=== | ||
| - | [[Image:Quorumsens.png|thumb|left| | + | [[Image:Quorumsens.png|thumb|left|300px|Fig 1 : Quorum sensing in ''V. fischeri''. (Red triangle represent AHL produced by LuxI. OM : Outer Membrane, IM : Inner Membrane]] |
In this system, LuxI and LuxR control the expression of luciferase operon ''luxICDABE'', necessary to produce bioluminescence. LuxI synthesize small diffusible molecules called Acyl Homoserin Lactone (AHL). LuxR is the intracellular receptor to AHL. | In this system, LuxI and LuxR control the expression of luciferase operon ''luxICDABE'', necessary to produce bioluminescence. LuxI synthesize small diffusible molecules called Acyl Homoserin Lactone (AHL). LuxR is the intracellular receptor to AHL. | ||
| - | The AHL freely diffuses into the extracellular medium. Its concentration increases as the population gets more dense. When the concentration reaches a critical level, those small molecules bind to their receptor. LuxR bound to AHL and the complex activate the operon that code for luciferase (''Figure 1''). | + | The AHL freely diffuses into the extracellular medium. Its concentration increases as the population gets more dense. When the concentration reaches a critical level, those small molecules bind to their receptor. LuxR bound to AHL and the complex activate the operon that code for luciferase (''Figure 1.''). |
===Bibliography=== | ===Bibliography=== | ||
Revision as of 17:53, 29 October 2008
Presentation of the phenomenon of quorum-sensing : intercellular communication in bacterias
The phenomenon of quorum sensing was first discovered in Vibrio fischeri. This bacteria only expresses luciferase when the density of bacteria is very high (1011bact/mL). Typically, the bacteria does not express bioluminescence when they are free living in the ocean. At the contrary, they express luciferase when they are highly concentrated in the photophore of Hawaiian bobtail squid.
The genetic cascade
In this system, LuxI and LuxR control the expression of luciferase operon luxICDABE, necessary to produce bioluminescence. LuxI synthesize small diffusible molecules called Acyl Homoserin Lactone (AHL). LuxR is the intracellular receptor to AHL.
The AHL freely diffuses into the extracellular medium. Its concentration increases as the population gets more dense. When the concentration reaches a critical level, those small molecules bind to their receptor. LuxR bound to AHL and the complex activate the operon that code for luciferase (Figure 1.).
Bibliography
x Christopher M Waters et Bonnie L Bassler, “Quorum sensing: cell-to-cell communication in bacteria,” Annual Review of Cell and Developmental Biology 21 (2005): 319-46, doi:10.1146/annurev.cellbio.21.012704.131001. xi A M Stevens, K M Dolan, et E P Greenberg, “Synergistic binding of the Vibrio fischeri LuxR transcriptional activator domain and RNA polymerase to the lux promoter region,” Proceedings of the National Academy of Sciences of the United States of America 91, no. 26 (Décembre 20, 1994): 12619-23, doi:7809088. xii Lingchong You et coll., “Programmed population control by cell-cell communication and regulated killing,” Nature 428, no. 6985 (Avril 22, 2004): 868-871, doi:10.1038/nature02491. xiii Frederick K Balagaddé et coll., “A synthetic Escherichia coli predator-prey ecosystem,” Molecular Systems Biology 4 (2008): 187, doi:msb200824
