Team:Chiba/Sender experiments/Senders(XL10Gold) T9002(JW1908)

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==Method==
 
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#Transformed Senders into E.coli strains(JW1908) and Receiver into E.coli strain(JW1908).
 
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#Inoculated them independently in liquid media. Incubated at 37°C 12h
 
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#Mixed them.
 
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#Incubated at 37°C.
 
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#Measured intensity of green fluorescence at regular time intervals.
 
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[[Team:Chiba/protocol/phenotype/timedelay|more details...]]
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==Results==
==Results==
===Reaction temparature:37°C===
===Reaction temparature:37°C===

Revision as of 17:53, 29 October 2008

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Results

Reaction temparature:37°C

  • センダーの培養液：500μL、レシーバの培養液：500μL
Fig.　　
E.coli strain,BBa_K084007:XL10Gold,BBa_T9002:JW1908,37°C,Receiver cells/Sender cells = 1.
Fig.　　
E.coli strain,BBa_K084007:XL10Gold,BBa_T9002:JW1908,37°C,Receiver cells/Sender cells = 1.
Fig.　　
E.coli strain,BBa_K084007:XL10Gold,BBa_T9002:JW1908,37°C,Receiver cells/Sender cells = 1.


Reaction temparature:30°C

  • センダーの培養液：500μL、レシーバの培養液：500μL
Fig.　　
E.coli strain,BBa_K084007:XL10Gold,BBa_T9002:JW1908,30°C,Receiver cells/Sender cells = 1.
Fig.　　
E.coli strain,BBa_K084007:XL10Gold,BBa_T9002:JW1908,30°C,Receiver cells/Sender cells = 1.


Reaction temparature:25°C

センダーの培養液：500μL、レシーバの培養液：500μL

Fig.
E.coli strain,Senders:XL10Gold,BBa_T9002:JW1908,25°C,Receiver cells/Sender cells = 1.
Fig.
　E.coli strain,Senders:XL10Gold,BBa_T9002:JW1908,25°C,Receiver cells/Sender cells = 1.


Left:

  1. 蛍光強度が、30°C,37°Cに比べて極端に低く,Sendersの培養液を添加しなかった反応液と差がほとんどなかった.LuxI,LasI,RhlI,LuxR,GFPすべての発現量が減っていたためと考えた.
  2. LuxI,RhlIの培養液を,混ぜた反応液に比べて,LasIの培養液を混ぜた反応液は,最大蛍光強度が小さかった(約1/2).

Right:

  1. RhlIにLVAtagのついているものは,LVAtagのついていないものに比べて最大蛍光強度が小さかった.



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