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Team:Chiba/Experiments:/LuxR mutant
From 2008.igem.org
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[[Image:Receiver switch mLuxR chiba.jpg|frame|left|'''Fig.''' LuxR mutant]] | [[Image:Receiver switch mLuxR chiba.jpg|frame|left|'''Fig.''' LuxR mutant]] | ||
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| + | == LuxR mutant (Under Plannning) == | ||
| + | ===Design=== | ||
| + | [[Image:Mutant-LuxR Chiba.gif|frame|right|'''Table.1''' Seisitivity of LuxR mutants. Data modified from Collins et.al. Mol. Microbiol. 55, 712–723 (2005)]] | ||
| + | [[Image:AHL-activation-of-mutagenizenized LuxR molecules Chiba.gif|frame|right|'''Table.Ahl activation of mutagenized LuxR molecules. Data modfied from B.Kock et al. Microbiology (2005), 151, 3589-3602''']] | ||
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| + | Collins et.al. described the hyper-sensitive variants of luxR to AHL.(Collins, C. H., Arnold, F. H. & Leadbetter, J. R. Directed evolution of Vibrio fischeri LuxR for increased sensitivity to a broad spectrum of acyl-homoserine lactones. Mol. Microbiol. 55, 712–723 | ||
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| + | LuxRに変異を入れることでLuxRのAHLに対する感受性を変えることができると報告されている。[[Team:Chiba/AHL Receiver Phase#References|<sup>(1),(2)</sup>]] | ||
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| + | #Ile45PheとするとAHLに対する感受性が約10倍上昇する[[Team:Chiba/AHL Receiver Phase#References|<sup>(1)</sup>]] | ||
| + | #Leu42AlaとするとAHLに対する感受性が1/15に減少する[[Team:Chiba/AHL Receiver Phase#References|<sup>(2)</sup>]] | ||
| + | #Leu42SerとするとAHLに対する感受性が1/1000に減少する[[Team:Chiba/AHL Receiver Phase#References|<sup>(2)</sup>]] | ||
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Revision as of 19:22, 29 October 2008
Contents |
LuxR/Plux mutants show
English:
日本語:私たちはmutated Receiverを用いることで、AHL感受性の違う２種類（WTと変異体）のレシーバーを用意し、delay-timeのバリエーションを増やした。
- a greater response to 3OC6HSL[http://authors.library.caltech.edu/5553/ (3)]
- a increase in sensitivity to 3OC12HSL[http://mic.sgmjournals.org/cgi/content/abstract/151/11/3589 (4)].
Receiver experiments details
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LuxR mutant (Under construction)
レシーバータンパク質であるLuxRに変異を入れることで応答感度を上下させる(5),(6)
Fig. LuxR mutant
LuxR mutant (Under Plannning)
Design
Table.1 Seisitivity of LuxR mutants. Data modified from Collins et.al. Mol. Microbiol. 55, 712–723 (2005)
Table.Ahl activation of mutagenized LuxR molecules. Data modfied from B.Kock et al. Microbiology (2005), 151, 3589-3602
Collins et.al. described the hyper-sensitive variants of luxR to AHL.(Collins, C. H., Arnold, F. H. & Leadbetter, J. R. Directed evolution of Vibrio fischeri LuxR for increased sensitivity to a broad spectrum of acyl-homoserine lactones. Mol. Microbiol. 55, 712–723
LuxRに変異を入れることでLuxRのAHLに対する感受性を変えることができると報告されている。(1),(2)
- Ile45PheとするとAHLに対する感受性が約10倍上昇する(1)
- Leu42AlaとするとAHLに対する感受性が1/15に減少する(2)
- Leu42SerとするとAHLに対する感受性が1/1000に減少する(2)
