Team:Chiba/Calendar-Home/12 September 2008

(Difference between revisions)

Revision as of 19:40, 29 October 2008

[http://partsregistry.org/Part:BBa_I1304 BBa_I1304]×1(1)(negative control)
[http://partsregistry.org/Part:BBa_R0051 BBa_R0051]×4(1)~(5)Select single colony from Agar Plate and introduce to 2mlLB each other.

Reaction mixtures were mixed in 48-well plate(deep well).

混ぜ表

Measured the Green Fluorescence intensity by Fluoroskan Ascent 2.5(program:Ascent Software Version 2.6).

L-アラビノースを入れてから15min(30min),1h,2h,6h,12h,24h後に蛍光強度を計測した。

Result:

Time after arabinose induction / Sample No.	1	2	3	4	5
30min	8.890	9.752	10.10	9.413	9.701
1h	9.161	13.93	17.37	14.97	10.60
2h	9.417	27.96	40.25	29.87	10.72
6h	9.933	74.99	150.9	125.0	10.04
12h	10.64	99.39	202.88	72.08	11.76
24h	14.66	105.6	447.3	33.31	17.11

no work

no work

@@ Line 7: / Line 7: @@
 *Pbad/araC:L-Arabinose induction test
-*BBa_I1304(Ptet-RBS-GFP,XL10G)×1(①)(negative control)
+*[http://partsregistry.org/Part:BBa_I1304 BBa_I1304]×1(1)(negative control)
-*BBa_R0051(PcI)×4(②～⑤)Select single colony from Agar Plate and introduce to 2mlLB each other.
+*[http://partsregistry.org/Part:BBa_R0051 BBa_R0051]×4(1)~(5)Select single colony from Agar Plate and introduce to 2mlLB each other.
@@ Line 37: / Line 37: @@
 Measured the Green Fluorescence intensity by Fluoroskan Ascent 2.5(program:Ascent Software Version 2.6).
-L-アラビノースを入れてから15min(?30min?),1h,2h,6h,12h,24h後に蛍光強度を計測した。
+L-アラビノースを入れてから15min(30min),1h,2h,6h,12h,24h後に蛍光強度を計測した。