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Template:Team:UC Berkeley/Notebook/SC notes
From 2008.igem.org
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Our busy schedule today: | Our busy schedule today: | ||
| - | We purified the PCR product (using Zymo cleanup); set up for restriction digest; cleaned up (Zymo) the digest; set up for Digestion; did Transformation; and we learned how to use the "spreader method" without setting the ethanol on fire... | + | We purified the PCR product (using Zymo cleanup); set up for restriction digest; cleaned up (Zymo) the digest; set up for Digestion; did Transformation; '''''and''''' we learned how to use the "spreader method" without setting the ethanol on fire... |
We finished at 8:15p.m. | We finished at 8:15p.m. | ||
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| + | == 26 June 2008 (Th) == | ||
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| + | It was a lighter day today: we're growing colonies overnight tonight, and will miniprep them tomorrow. | ||
| + | Since our schedule was less packed today, we also did a couple minipreps for Madhvi. | ||
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Revision as of 00:32, 27 June 2008
Contents |
23 June 2008 (M)
Having completed one week of training/lectures, I've finally started on my minute portion of the lab work! Over last weekend, I searched for the DNA sequences of the FLAG-tag and the AP-tag. The objective was to find an existing E. coli vector for said sequences. Upon completing the search, I designed oligos for EIPCR for small parts under 30 bp (like our tags). When we discovered, however, that our sequences were too long - leaning precariously over the "30bp border" - we decided to prepare for a Wobble (overlap extension) PCR instead. This involved designing Fwd/Rev oligos that had a 20bp overlap. When that was done, we ordered the oligos. We now eagerly expect their arrival tomorrow at around 3p.m., when we can actually do the PCR reation!
24 June 2008 (T)
I finished creating/organizing my oligos and construction files onto spreadsheets and documents.
We did set up for the wobble PCRs; they're in the thermocycler overnight until tomorrow!
25 June 2008 (W)
Our busy schedule today:
We purified the PCR product (using Zymo cleanup); set up for restriction digest; cleaned up (Zymo) the digest; set up for Digestion; did Transformation; and we learned how to use the "spreader method" without setting the ethanol on fire...
We finished at 8:15p.m.
26 June 2008 (Th)
It was a lighter day today: we're growing colonies overnight tonight, and will miniprep them tomorrow. Since our schedule was less packed today, we also did a couple minipreps for Madhvi.
