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Team:Chiba/Calendar-Home/23 August 2008
From 2008.igem.org
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| Line 40: | Line 40: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td>DNA</td> | + | <td>DNA(μL)</td> |
<td>3</td><td>1</td><td>3</td><td>3</td> | <td>3</td><td>1</td><td>3</td><td>3</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td>EcoRI | + | <td>EcoRI(μL)td> |
<td>-</td><td>-</td><td>0.1</td><td>0.1</td> | <td>-</td><td>-</td><td>0.1</td><td>0.1</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td>PstI</td> | + | <td>PstI(μL)</td> |
<td>-</td><td>-</td><td>0.1</td><td>0.1</td> | <td>-</td><td>-</td><td>0.1</td><td>0.1</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td>Buffer EcoRI</td> | + | <td>Buffer EcoRI(μL)</td> |
<td>-</td><td>-</td><td>0.9</td><td>0.8</td> | <td>-</td><td>-</td><td>0.9</td><td>0.8</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td>BSA</td> | + | <td>BSA(μL)</td> |
<td>-</td><td>-</td><td>1</td><td>1</td> | <td>-</td><td>-</td><td>1</td><td>1</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td>dH<sub>2</sub>O</td> | + | <td>dH<sub>2</sub>O(μL)</td> |
<td>-</td><td>-</td><td>5</td><td>5</td> | <td>-</td><td>-</td><td>5</td><td>5</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td>TOTAL</td> | + | <td>TOTAL(μL)</td> |
| - | <td> | + | <td>3</td><td>1</td><td>10</td><td>10</td> |
</tr> | </tr> | ||
</table> | </table> | ||
| Line 85: | Line 85: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td>Loading Dye</td> | + | <td>Loading Dye(μL)</td> |
<td>1</td><td>1</td> | <td>1</td><td>1</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td>dH<sub>2</sub>O</td> | + | <td>dH<sub>2</sub>O(μL)</td> |
<td>2</td><td>4</td> | <td>2</td><td>4</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td>TOTAL</td> | + | <td>TOTAL(μL)</td> |
| - | <td> | + | <td>6</td><td>6</td> |
</tr> | </tr> | ||
</table> | </table> | ||
| Line 120: | Line 120: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td>Sample DNA</td> | + | <td>Sample DNA(μL)</td> |
<td>10</td> | <td>10</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td>Loading Dye</td> | + | <td>Loading Dye(μL)</td> |
<td>2</td> | <td>2</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td>TOTAL</td> | + | <td>TOTAL(μL)</td> |
| - | <td> | + | <td>12</td> |
</tr> | </tr> | ||
</table> | </table> | ||
| Line 165: | Line 165: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td>Sample DNA</td> | + | <td>Sample DNA(μL)</td> |
<td>3</td><td>3</td><td>3</td> | <td>3</td><td>3</td><td>3</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td>EcoRI</td> | + | <td>EcoRI(μL)</td> |
<td>-</td><td>0.1</td><td>0.1</td> | <td>-</td><td>0.1</td><td>0.1</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td>SpeI</td> | + | <td>SpeI(μL)</td> |
<td>-</td><td>-</td><td>0.1</td> | <td>-</td><td>-</td><td>0.1</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td>EcoRI Buffer</td> | + | <td>EcoRI Buffer(μL)</td> |
<td>-</td><td>0.9</td><td>0.8</td> | <td>-</td><td>0.9</td><td>0.8</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td>BSA</td> | + | <td>BSA(μL)</td> |
<td>-</td><td>1</td><td>1</td> | <td>-</td><td>1</td><td>1</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td>dH<sub>2</sub>O</td> | + | <td>dH<sub>2</sub>O(μL)</td> |
<td>-</td><td>5</td><td>5</td> | <td>-</td><td>5</td><td>5</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td>TOTAL</td> | + | <td>TOTAL(μL)</td> |
<td>3</td><td>10</td><td>10</td> | <td>3</td><td>10</td><td>10</td> | ||
</tr> | </tr> | ||
| Line 209: | Line 209: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td>Loading Dye</td> | + | <td>Loading Dye(μL)</td> |
<td>1</td><td>2</td><td>2</td> | <td>1</td><td>2</td><td>2</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td>dH<sub>2</sub>O</td> | + | <td>dH<sub>2</sub>O(μL)</td> |
<td>2</td><td>-</td><td>-</td> | <td>2</td><td>-</td><td>-</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td>TOTAL</td> | + | <td>TOTAL(μL)</td> |
| - | <td> | + | <td>6</td><td>12</td><td>12</td> |
</tr> | </tr> | ||
</table> | </table> | ||
Revision as of 06:01, 30 October 2008
22 August 2008 <|> 24 August 2008
Contents |
Meeting with UmeG
the ppt for our weekly meeting(japanese)
- Team:Input
- Team:Communication
- team:Output
Laboratory work
Team:Input
no work
Team:Communication
- (21/8)--->Mini prep
- [http://partsregistry.org/Part:BBa_S03154 BBa_S03154](2007)
- [http://partsregistry.org/Part:BBa_S03154 BBa_S03154](2006)
- [http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2007)
- [http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2006)
- [http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2007)
- [http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2006)
- --->Digestion test
- [http://partsregistry.org/Part:BBa_S03154 BBa_S03154](2007)
- [http://partsregistry.org/Part:BBa_S03154 BBa_S03154](2006)
- [http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2007)
- [http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2006)
- [http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2007)
- [http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2006)
- [http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007)
Sample No. 1- 6 7 1 - 7 1 - 7 DNA(μL) 3 1 3 3 EcoRI(μL)td> - - 0.1 0.1 PstI(μL) - - 0.1 0.1 Buffer EcoRI(μL) - - 0.9 0.8 BSA(μL) - - 1 1 dH2O(μL) - - 5 5 TOTAL(μL) 3 1 10 10
- --->Gel Check
Sample No. 1~6 7 Sample DNA 3 1 Loading Dye(μL) 1 1 dH2O(μL) 2 4 TOTAL(μL) 6 6 - From left; Sanple No. 1~7
- [http://partsregistry.org/Part:BBa_S03154 BBa_S03154](2007)
- [http://partsregistry.org/Part:BBa_S03154 BBa_S03154](2006)
- [http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2007)
- [http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2006)
- [http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2007)
- [http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2006)
- [http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007)
- From left; Sanple No. 1~7
Sample No. 8~21 Sample DNA(μL) 10 Loading Dye(μL) 2 TOTAL(μL) 12 - From left;
- (Single Digestion) Sanple No. 8~14
- [http://partsregistry.org/Part:BBa_S03154 BBa_S03154](2007) -> OK
- [http://partsregistry.org/Part:BBa_S03154 BBa_S03154](2006) -> OK
- [http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2007) -> OK
- [http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2006) -> OK
- [http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2007) -> OK
- [http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2006) -> OK
- [http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007) -> OK
- (Double Digestion) Sample No. 15~21
- [http://partsregistry.org/Part:BBa_S03154 BBa_S03154](2007) -> OK
- [http://partsregistry.org/Part:BBa_S03154 BBa_S03154](2006) -> OK
- [http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2007) -> OK
- [http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2006) -> OK
- [http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2007) -> OK
- [http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2006) -> OK
- [http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007) -> OK?
- Digestion test
- [http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007:old-sample)
Sample No. 1 2 3 Sample DNA(μL) 3 3 3 EcoRI(μL) - 0.1 0.1 SpeI(μL) - - 0.1 EcoRI Buffer(μL) - 0.9 0.8 BSA(μL) - 1 1 dH2O(μL) - 5 5 TOTAL(μL) 3 10 10
--->Gel Check
Sample No. 1 2 3 Sample DNA 3 10 10 Loading Dye(μL) 1 2 2 dH2O(μL) 2 - - TOTAL(μL) 6 12 12 - From left;
- [http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007) -> OK
- [http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007)Single Digestion -> OK
- [http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007)Double Digestion -> Bad
- From left;
Team:Output
no work
