"
Genetic Circuit
From 2008.igem.org
(Difference between revisions)
| Line 26: | Line 26: | ||
The diagram of the network is shown as well. | The diagram of the network is shown as well. | ||
| - | [[Image:Peking hAID final.jpg]] | + | [[Image:Peking hAID final.jpg|500px]] |
To simplify the model, we combine the transcription and replication to one process of DNA expression. Also introduce the Hill-equation to demonstrate the function of repression and activation of the transcriptional factors. Other technical procedure introduced as well, we simplify the model further. The simplified model is as below. | To simplify the model, we combine the transcription and replication to one process of DNA expression. Also introduce the Hill-equation to demonstrate the function of repression and activation of the transcriptional factors. Other technical procedure introduced as well, we simplify the model further. The simplified model is as below. | ||
| - | [[Image:Peking hAID simplified.jpg]] | + | [[Image:Peking hAID simplified.jpg|500px]] |
{|align="right" | {|align="right" | ||
|[[Image:Peking_legend.jpg|200px]] | |[[Image:Peking_legend.jpg|200px]] | ||
Revision as of 03:54, 30 October 2008
Part II Genetic Circuit
Under Matlab Simbiology Toolbox, we demonstrate all the reactions listed below.
SimBiology Reaction Array
Index: Reaction: 1 DNA.lacO_hAID_lexA + Pro.LacI <-> DNA.[lacO:LacI] 2 DNA.lacO_hAID_lexA -> mRNA.lacO_hAID_lexA + DNA.lacO_hAID_lexA 3 mRNA.lacO_hAID_lexA -> null 4 mRNA.lacO_hAID_lexA -> Pro.hAID_LexA 5 Pro.hAID_LexA + DNA.DeGal4_lexO <-> DNA.[hAID_LexA:DeGal4_lexO] 6 Pro.hAID_LexA -> null 7 DNA.Gal4_LexO -> mRNA.Gal4_LexO + DNA.Gal4_LexO 8 mRNA.Gal4_LexO -> Pro.Gal4 9 Pro.Gal4 + DNA.UAS_his_lacZ_lacI -> DNA.[Gal4:UAS_his_lacZ_lacI] 10 DNA.[Gal4:UAS_his_lacZ_lacI] -> mRNA.his_lacZ_LacI 11 mRNA.his_lacZ_LacI -> Pro.LacI + Pro.His + Pro.LacZ 12 mRNA.Gal4_LexO -> null 13 mRNA.his_lacZ_LacI -> null 14 Pro.LacI -> null 15 Pro.Gal4 -> null 16 DNA.[hAID_LexA:Gal4_lexO] -> Pro.hAID_LexA + DNA.Gal4_LexO 17 DNA.[hAID_LexA:DeGal4_lexO] -> DNA.[hAID_LexA:Gal4_lexO] 18 Pro.His -> null 19 Pro.LacZ -> null
The diagram of the network is shown as well.
To simplify the model, we combine the transcription and replication to one process of DNA expression. Also introduce the Hill-equation to demonstrate the function of repression and activation of the transcriptional factors. Other technical procedure introduced as well, we simplify the model further. The simplified model is as below.
|
| Previous Page |
