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Team:Chiba/Calendar-Home/26 September 2008
From 2008.igem.org
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</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td>dH<sub>2</sub>O(μL)</td> |
<td>19.8</td> | <td>19.8</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td>TOTAL</td> | + | <td>TOTAL(μL)</td> |
<td>30</td> | <td>30</td> | ||
</tr> | </tr> | ||
Revision as of 20:12, 29 October 2008
25 September 2008 <|> 27 September 2008
Laboratory work
Team:Communication
Team:Output
TIME DELAY TEST
colony PCR
Ligation product(26,September)×7
Vector:BBa_R0062
| Sample No. | each |
| culture(μL) | 1 |
| Fwd primer(μL) | 1.5 |
| Rev primer(μL) | 1.5 |
| Taq(DNA pol)(μL) | 0.2 |
| dNTPmix(μL) | 3 |
| thermo pol Buffer(μL) | 3 |
| dH2O(μL) | 19.8 |
| TOTAL(μL) | 30 |
->95°C 5 min ->(95°C 1 min 50°C 30 sec 72°C 1 min)×25 ->72°C 10 min
->Gel check
->MINI Prep
