Team:Imperial College/Major Results

From 2008.igem.org

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{{Imperial/Box1|Our Work With ''B. subtilis'' Chassis|
{{Imperial/Box1|Our Work With ''B. subtilis'' Chassis|
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[https://2008.igem.org/Team:Imperial_College/B.subtilis Summary Page]
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[https://2008.igem.org/Team:Imperial_College/B.subtilis Please click here for more information]
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B.subtilis has great potential as a chassis for synthetic biology. However it is not commonly used in iGEM competition and very few B.subtilis BioBricks are available. In order to increase the accessibility of B.subtilis as a chassis, we have created 45 new BioBricks, successfully transformed B.subtilis and characterised their growth.
B.subtilis has great potential as a chassis for synthetic biology. However it is not commonly used in iGEM competition and very few B.subtilis BioBricks are available. In order to increase the accessibility of B.subtilis as a chassis, we have created 45 new BioBricks, successfully transformed B.subtilis and characterised their growth.
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{{Imperial/Box1|Characterisation of BioBricks|
{{Imperial/Box1|Characterisation of BioBricks|
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[https://2008.igem.org/Team:Imperial_College/Biobricks '''Please Click Here For the Biobricks Characterisation Summary Page''']<br>
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[https://2008.igem.org/Team:Imperial_College/Biobricks '''Please click here For the BioBricks Characterisation Summary Page''']<br>
'''We have characterised several of our testing constructs in ''B.stubilis''.This has shown that a variety of our parts are functional including integration sites, antibiotic resistance, promoters and ribosome binding sites.'''
'''We have characterised several of our testing constructs in ''B.stubilis''.This has shown that a variety of our parts are functional including integration sites, antibiotic resistance, promoters and ribosome binding sites.'''
|<html><a target="_blank" href="https://static.igem.org/mediawiki/2008/b/b7/Fluorescence_B.subtilis.PNG"><img width="200px" src="https://static.igem.org/mediawiki/2008/b/b7/Fluorescence_B.subtilis.PNG"></a></html>
|<html><a target="_blank" href="https://static.igem.org/mediawiki/2008/b/b7/Fluorescence_B.subtilis.PNG"><img width="200px" src="https://static.igem.org/mediawiki/2008/b/b7/Fluorescence_B.subtilis.PNG"></a></html>

Revision as of 21:35, 29 October 2008


Experimental Results

Major results are shown in this section; this page will be updated shortly. For minor results, please refer to our Notebook.


Our Work With B. subtilis Chassis

Please click here for more information
B.subtilis has great potential as a chassis for synthetic biology. However it is not commonly used in iGEM competition and very few B.subtilis BioBricks are available. In order to increase the accessibility of B.subtilis as a chassis, we have created 45 new BioBricks, successfully transformed B.subtilis and characterised their growth.


Control of Motility

Summary Page

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Characterisation of Chloramphenicol Acetyltransferase


Please Click Here For the Chloramphenicol acetyltransferase Characterisation Summary Page
We have characterised the ability of Chloramphenicol acetyltransferase([http://partsregistry.org/wiki/index.php?title=Part:BBa_J31005 BBa_J31005]) to provide resistance to Chlroamphenicol. This has shown that a variety of our parts are functional including integration sites, promoters and ribosome binding sites and the range of chloramphenicol concentrations at which a cell will be protected by Chloramphenicol acetyltransferase.


Characterisation of BioBricks

Please click here For the BioBricks Characterisation Summary Page
We have characterised several of our testing constructs in B.stubilis.This has shown that a variety of our parts are functional including integration sites, antibiotic resistance, promoters and ribosome binding sites.




General lab work and smaller results are put up on the calendar on the dates they were done; they can therefore be found in the >>> Notebook >>>



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