Purdue/27 June 2008

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The "transformed" cells still did not grow on an Amp plate, meaning no cells took up the LacZ or the SOS DNA.  The problem is most likely the DNA and/or the DNA prepping procedure.  We'll need to contact our iGEM contact, and ask what to do.
The "transformed" cells still did not grow on an Amp plate, meaning no cells took up the LacZ or the SOS DNA.  The problem is most likely the DNA and/or the DNA prepping procedure.  We'll need to contact our iGEM contact, and ask what to do.
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Later today, we'll inoculate another liquid media tube (so as not to lose the stock of cells), and plate again on newer Amp plates (to see if the old agar was too old or something).
'''Edited by Janie Stine'''
'''Edited by Janie Stine'''

Revision as of 13:54, 27 June 2008

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The "transformed" cells still did not grow on an Amp plate, meaning no cells took up the LacZ or the SOS DNA. The problem is most likely the DNA and/or the DNA prepping procedure. We'll need to contact our iGEM contact, and ask what to do.

Later today, we'll inoculate another liquid media tube (so as not to lose the stock of cells), and plate again on newer Amp plates (to see if the old agar was too old or something).

Edited by Janie Stine