Team:Imperial College/CAT

From 2008.igem.org

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=Chloramphenicol Acetyltransferase Characterisation=
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{{Imperial/StartPage2}}
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===Chloramphenicol Acetyltransferase Characterisation===
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Characterisation of the chloramphenicol concentrations at which chlorampehnicol acetyltransferase ([http://partsregistry.org/wiki/index.php?title{{equals}}Part:BBa_J31005 Part: BBa_J31005]) (CAT) will protect cells, allowing them to grow and thrive is essentially to allow the correct selection when using the CAT gene.
Characterisation of the chloramphenicol concentrations at which chlorampehnicol acetyltransferase ([http://partsregistry.org/wiki/index.php?title{{equals}}Part:BBa_J31005 Part: BBa_J31005]) (CAT) will protect cells, allowing them to grow and thrive is essentially to allow the correct selection when using the CAT gene.
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To this end, characterisation of chloramphenicol acetyl transferase was carried out according to this [https://2008.igem.org/wiki/index.php?title=Team:Imperial_College/Resistance protocol]
To this end, characterisation of chloramphenicol acetyl transferase was carried out according to this [https://2008.igem.org/wiki/index.php?title=Team:Imperial_College/Resistance protocol]
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==Results==
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[Image:CAT_Results.PNG|width=80%]
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[Image:CAT_Working_Concentration.PNG|width=80%]
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The construct containing the CAT gene was integrate into the ''B. subtilis'' genome for characterisation. As such, the CAT gene is single copy only. CAT will be able to protect a cell from higher levels of chloramphenicol if it is present in a construct on a plasmid (particularly a high copy number plasmid)
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<br><hr>{{Imperial/EndPage|Major_Results|Major_Results}}

Revision as of 22:56, 29 October 2008


Chloramphenicol Acetyltransferase Characterisation


Results

[Image:CAT_Results.PNG