Team:Imperial College/Biobricks

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Revision as of 21:40, 29 October 2008

Biobrick Characterisation in B.subtilis
This year Imperial College has added 45 new B.subtilis specific parts to the registry. In addition we sort to characterise some of these parts, including the constitutive promoter and ribosome binding site pveg-spoVG. The testing constructs we developed for this are shown below:

Summary of Data

The promoter and RBS combinations were characterised by measuring the expression of fluorescent proteins in B.subtilis. Cultures of B.subtilis transformed with the test constructs and non-transformed B.subtilis (control) were grown to the mid-log phase. Fluorescence and O.D.₆₀₀ were both measured using a plate reader to generate fluorescence levels of the various samples. To make this data more generic the fluorescence data was normalized based on cell number using the O.D.₆₀₀ and a calibration curve of O.D.₆₀₀ vs colony forming units, as explained in the diagram below:

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 {{Imperial/Box1|Summary of Data|
-The promoter and RBS combinations were characterised by measuring the steadty-state levels expression of fluorescent proteins in ''B.subtilis''. Cultures of ''B.subtilis'' transformed with the test constructs and non-transformed ''B.subtilis'' (control) were grown to the mid-log phase. Fluorescence and O.D.<sub>600</sub> were both measured using a plate reader to generate fluorescence levels of the various samples. This fluorescence data was normalised based on cell density by using the O.D.<sub>600</sub> and a '''calibration curve of O.D.<sub>600</sub> vs colony forming units'''
+The promoter and RBS combinations were characterised by measuring the expression of fluorescent proteins in ''B.subtilis''. Cultures of ''B.subtilis'' transformed with the test constructs and non-transformed ''B.subtilis'' (control) were grown to the mid-log phase. Fluorescence and O.D.<sub>600</sub> were both measured using a plate reader to generate fluorescence levels of the various samples. To make this data more generic the fluorescence data was normalized based on cell number using the O.D.<sub>600</sub> and a '''calibration curve of O.D.<sub>600</sub> vs colony forming units''', as explained in the diagram below:
+<br>
+[[Image:Calibration Picture.PNG|600px|center]]