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Team:Chiba/Calendar-Home/8 September 2008
From 2008.igem.org
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'''[[Team:Chiba/protocol/ligation/gelex|Gel Extract]]''' | '''[[Team:Chiba/protocol/ligation/gelex|Gel Extract]]''' | ||
| + | {|align="justify" | ||
| + | |[[Image:Chiba-0908.JPG|300px]] | ||
| + | | | ||
| + | :<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000"> | ||
| + | <tr> | ||
| + | <td width="257">Sample DNA</td> | ||
| + | <td>60</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>Loading Dye</td> | ||
| + | <td>12</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>TOTAL</td> | ||
| + | <td>72μL</td> | ||
| + | </tr> | ||
| + | </table> | ||
| + | :From left; | ||
| + | ::[http://partsregistry.org/Part:BBa_ BBa_](2007) | ||
| + | ::100 ng / 1 well | ||
| + | |} | ||
| + | |||
| + | {|align="justify" | ||
| + | |[[Image:Chiba-0908-2.JPG]] | ||
| + | | | ||
| + | :<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000"> | ||
| + | <tr> | ||
| + | <td width="257">Sample DNA</td> | ||
| + | <td>30</td><td>60</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>Loading Dye</td> | ||
| + | <td>6</td><td>12</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>TOTAL</td> | ||
| + | <td>36μL</td><td>72μL</td> | ||
| + | </tr> | ||
| + | </table> | ||
| + | :From left; | ||
| + | ::[http://partsregistry.org/Part:BBa_J04500 BBa_J04500] | ||
| + | ::[http://partsregistry.org/Part:BBa_C0161 BBa_C0161] | ||
| + | |} | ||
| + | |||
| + | {|align="justify" | ||
| + | |[[Image:Chiba-0908-3.JPG]] | ||
| + | | | ||
| + | ::[http://partsregistry.org/Part:BBa_C0261 BBa_C0261] -> none | ||
| + | |} | ||
Revision as of 22:37, 29 October 2008
7 September 2008 <|> 9 September 2008
Contents |
Laboratory work
Team:Input
no work
Team:Communication
-->(7/9)
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- --->Zymo Clean
- eluted with 15μL(?) of NFW.
Team:Output
- [http://partsregistry.org/Part:BBa_R0040 BBa_R0040](1)
- [http://partsregistry.org/Part:BBa_R0010 BBa_R0010](5)
| Sample No. | 1 | 5 | |
| DNA(digested sample)(μL) | 7 | 6 | |
| SAP(μL) | 2 | 2 | |
| SAP Buffer(μL) | 3 | 3 | |
| TOTAL(μL) | 30 | 30 |
-->left at 37 degrees for 1 hour.
-->left at 65 degrees for 30 minutes.
-->DNA purification(1:21μl,5:6μl)
-->Gel check
| Sample No. | each |
| DNA(digested sample)(μL) | 1 |
| Loading Dye(μL) | 1 |
| dH2O(μL) | 4 |
| TOTAL(μL) | 6 |
- vector:[http://partsregistry.org/Part:BBa_R0040 BBa_R0040](1) 75ng/μl
- insert:[http://partsregistry.org/Part:BBa_S03158 BBa_S03158](2) 33.6ng/μl
- insert:[http://partsregistry.org/Part:BBa_S03156 BBa_S03156](3) 33.6ng/μl
- insert:[http://partsregistry.org/Part:BBa_S03160 BBa_S03160](4) 38.4ng/μl
- vector:[http://partsregistry.org/Part:BBa_R0010 BBa_R0010](5) 100ng/μl
- insert:[http://partsregistry.org/Part:BBa_J52008 BBa_J52008](6) 33.6ng/μl
| Sample No. | 1&2 | 1&3 | 1&4 | 1 | 5&6 | 5 |
| DNA(vector)(μL) | 3 | 3 | 3 | 3 | 5 | 2.1 |
| DNA(insert)(μL) | 3 | 3 | 3 | - | 3.1 | - |
| Ligase(μL) | 1 | 1 | 1 | 1 | 1 | 1 |
| Ligase Buffer(x5)(μL) | 2 | 2 | 2 | 2 | 2 | 2 |
| dH2O(μL) | 1 | 1 | 1 | 4 | 8.9 | 4 |
| TOTAL(μL) | 10 | 10 | 10 | 10 | 20 | 10 |
