Team:ETH Zurich/Project/Approach

From 2008.igem.org

(Difference between revisions)

Revision as of 00:52, 30 October 2008

Our Approach

We are following an approach based on random deletions a designed selection mechanism to reach our goal. As mentioned in the Motivation section, we postulate that random disruption of genes combined with an evolutionary approach

In order to prove the viability of the concept, we intend to demonstrate that .. a) .. excision of genomic sequences by restriction enzymes can be performed within the living cell; b) .. fragmented genomic DNA can be ligated inside the living cell by simultaneous expression of T4 ligase; c) .. pulse generators that produce pulses of protein expression of variable length terminated by induction with a second compound can be devised both on the transcriptional as well as on the translational level; d) .. cells with optimized growth and reproduction can be selected by competition inside chemostat cultures.

@@ Line 13: / Line 13: @@
 == Our Approach ==
-[[Image:approach.png]]
+We are following an approach based on random deletions a designed selection mechanism to reach our goal. As mentioned in the [[Team:ETH Zurich/Project/Motivation|Motivation]] section, we postulate that random disruption of genes combined with an evolutionary approach
+In order to prove the viability of the concept, we intend to demonstrate that ..
+a) .. excision of genomic sequences by restriction enzymes can be performed within the living cell;
+b) .. fragmented genomic DNA can be ligated inside the living cell by simultaneous expression of T4 ligase;
+c) .. pulse generators that produce pulses of protein expression of variable length terminated by induction with a second compound can be devised both on the transcriptional as well as on the translational level;
+d) .. cells with optimized growth and reproduction can be selected by competition inside chemostat cultures.
+[[Image:approach.png]]
 == Random walk towards a minimal genome ==