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Team:Chiba/Calendar-Home/16 September 2008
From 2008.igem.org
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===Team:Output=== | ===Team:Output=== | ||
Latest revision as of 00:00, 30 October 2008
15 September 2008 <|> 17 September 2008
Laboratory work
Team:Communication
time-delay test with (15/9) cultures.
- reaction temparature was 37°C and 30°C.
- result
- Created Glycerol Stocks from (15/9) cultures.
Team:Output
Digestion test
- [http://partsregistry.org/wiki/index.php?title=Part:BBa_K084004 BBa_K084004](×2)
- [http://partsregistry.org/wiki/index.php?title=Part:BBa_K084005 BBa_K084005](×5)
- [http://partsregistry.org/wiki/index.php?title=Part:BBa_K084006 BBa_K084006](×2)
- [http://partsregistry.org/Part:BBa_S03158 BBa_S03158]
- [http://partsregistry.org/Part:BBa_S03156 BBa_S03156]
- [http://partsregistry.org/Part:BBa_S03160 BBa_S03160]
| Sample No. | each |
| DNA(μL) | 3 |
| EcoRI(μL) | 0.083 |
| PstI(μL) | 0.083 |
| BSA(×10)(μL) | 1 |
| Buffer3(×10)(μL) | 1 |
| dH2O(μL) | 5 |
| TOTAL(μL) | 10 |
-->37°C 1 hour
| Sample No. | each |
| DNA(μL) | 10 |
| Loading Dye(μL) | 2 |
| TOTAL(μL) | 12 |
- [http://partsregistry.org/Part:BBa_J37019 BBa_J37019](LuxI+RBS+LuxR)->no colony
- [http://partsregistry.org/Part:BBa_J37033 BBa_J37033](RBS+LuxR)
- [http://partsregistry.org/Part:BBa_J37020 BBa_J37020](LuxI+LuxR+pLux+GFP)
- [http://partsregistry.org/Part:BBa_J37032 BBa_J37032](pLux+GFP)
- [http://partsregistry.org/wiki/index.php?title=Part:BBa_K084003 BBa_K084003]
| Sample No. | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K084003 BBa_K084003] |
| DNA(μL) | 1 |
| Venus Fwd(μL) | 5 |
| Rev(VR)(μL) | 5 |
| Thermo pol Buffer(μL) | 10 |
| dNTP mix(μL) | 10 |
| Vent(μL) | 1 |
| dH2O(μL) | 68 |
| TOTAL(μL) | 100 |
