Team:Chiba/Calendar-Home/17 September 2008

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(→Team:Output)
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===Team:Output===
===Team:Output===
[[Team:Chiba/protocol/transformation|Transformed]]
[[Team:Chiba/protocol/transformation|Transformed]]
-
*[http://partsregistry.org/Part:BBa_I13500 BBa_I13500](RBS+GFP)
+
*[http://partsregistry.org/Part:BBa_I13500 BBa_I13500]
-
*[http://partsregistry.org/Part:BBa_J37019 BBa_J37019](Luxpr+LuxR)-->no colony
+
*[http://partsregistry.org/Part:BBa_J37019 BBa_J37019]-->no colony
[[Team:Chiba/protocol/DNA_Purification/sigma|miniprepped]]
[[Team:Chiba/protocol/DNA_Purification/sigma|miniprepped]]
-
*[http://partsregistry.org/Part:BBa_J37033 BBa_J37033](RBS+LuxR)
+
*[http://partsregistry.org/Part:BBa_J37033 BBa_J37033]
-
*[http://partsregistry.org/Part:BBa_J37020 BBa_J37020](Luxpr+LuxR+LuxR+GFP)
+
*[http://partsregistry.org/Part:BBa_J37020 BBa_J37020]
-
*[http://partsregistry.org/Part:BBa_J37032 BBa_J37032](pLux+GFP)
+
*[http://partsregistry.org/Part:BBa_J37032 BBa_J37032]
[[Team:Chiba/protocol/digestion|Digestion]]
[[Team:Chiba/protocol/digestion|Digestion]]
-
*[http://partsregistry.org/Part:BBa_I7100 BBa_I7100](ptetR+RBS)
+
*[http://partsregistry.org/Part:BBa_I7100 BBa_I7100]
-
*[http://partsregistry.org/Part:BBa_S03119 BBa_S03119](ptet+LuxR)
+
*[http://partsregistry.org/Part:BBa_S03119 BBa_S03119]
*[http://partsregistry.org/wiki/index.php?title=Part:BBa_K084004 BBa_K084004]
*[http://partsregistry.org/wiki/index.php?title=Part:BBa_K084004 BBa_K084004]
*[http://partsregistry.org/wiki/index.php?title=Part:BBa_K084005 BBa_K084005]
*[http://partsregistry.org/wiki/index.php?title=Part:BBa_K084005 BBa_K084005]

Latest revision as of 22:35, 29 October 2008

>Home | Notebook

16 September 2008 <|> 18 September 2008

Laboratory work

Team:Communication

  • time-delay test
    • transformants
      • [http://partsregistry.org/Part:BBa_S03623 BBa_S03623]
      • [http://partsregistry.org/Part:BBa_K084007 BBa_K084007]
      • [http://partsregistry.org/Part:BBa_K084008 BBa_K084008]
      • [http://partsregistry.org/Part:BBa_K084009 BBa_K084009]
    • E.coli strain
      Senders:JW1908
      Receiver:JW1908
    • reaction temparature
      37°C and 30°C


  • time-delay test
    • transformants
      • [http://partsregistry.org/Part:BBa_S03623 BBa_S03623]
      • [http://partsregistry.org/Part:BBa_K084007 BBa_K084007]
      • [http://partsregistry.org/Part:BBa_K084008 BBa_K084008]
    • E.coli strain
      Senders:XL10GOLD
      Receiver:JW1908
    • reaction temparature
      37°C and 30°C

Team:Output

Transformed

  • [http://partsregistry.org/Part:BBa_I13500 BBa_I13500]
  • [http://partsregistry.org/Part:BBa_J37019 BBa_J37019]-->no colony

miniprepped

  • [http://partsregistry.org/Part:BBa_J37033 BBa_J37033]
  • [http://partsregistry.org/Part:BBa_J37020 BBa_J37020]
  • [http://partsregistry.org/Part:BBa_J37032 BBa_J37032]

Digestion

  • [http://partsregistry.org/Part:BBa_I7100 BBa_I7100]
  • [http://partsregistry.org/Part:BBa_S03119 BBa_S03119]
  • [http://partsregistry.org/wiki/index.php?title=Part:BBa_K084004 BBa_K084004]
  • [http://partsregistry.org/wiki/index.php?title=Part:BBa_K084005 BBa_K084005]
  • [http://partsregistry.org/wiki/index.php?title=Part:BBa_K084006 BBa_K084006]
Sample No. each
DNA template(μL) 25
EcoRI(μL) 1
PstI(μL) 1
BSA(×10)(μL) 4
EcoRI Buffer(μL) 4
dH2O(μL) 5
TOTAL(μL) 40

-->Left at 37 °C for 1 hour.

-->Next day

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