From 2008.igem.org
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- | === PCR Verification of Recombinant ===
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Revision as of 23:44, 29 October 2008
OUR TEAM :::
SUMMARY ::: INTRODUCTION ::: STRATEGY ::: RESULTS ::: ONGOING WORK ::: GALLERY
Construction
Notebook
Constructs
Yeast Transformation
HPLC Data
To analyze our beer samples for resveratrol content, be will be using High Performance Liquid Chromatography (HPLC), which will allow us to separate the metabolites produced by the yeast and analyze these compounds by spectrophotometry. By comparing HPLC chromatogram peaks of metabolites produced by our yeast with a resveratrol-only standard, we can identify if resveratrol is being produced, and at what quantities. Below, we show our initial data for HPLC calibration curves with known quantities of resveratrol and p-coumaric acid standards and test chromatograms using extracts from different wine samples.
HPLC: Calibration
HPLC: Fermenation batches
Fermentation
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SUMMARY ::: BACKGROUND ::: STRATEGY ::: CONSTRUCTS ::: RESULTS ::: ONGOING WORK ::: OUR TEAM ::: NOTEBOOK ::: GALLERY
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