Template:Team:UC Berkeley/Notebook/CDB construction

From 2008.igem.org

(Difference between revisions)
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cb0005-fabA-F Forward Biobricking  cgataGAATTCatgAGATCTggccattacgttggctgaac  
cb0005-fabA-F Forward Biobricking  cgataGAATTCatgAGATCTggccattacgttggctgaac  
cb0006-fabA-R Reverse Biobricking  cgtagGGATCCgaagttaccacccgtttcgg
cb0006-fabA-R Reverse Biobricking  cgtagGGATCCgaagttaccacccgtttcgg
 +
</pre>
 +
 +
==pBca1256-K112403==
 +
<pre>
 +
Biobricking of signal peptide (prepro) of phoA (alkaline phosphatase)
 +
PCR cb10007-F and cb10008 on MG1655 (114 bp, EcoRI/BamHI)
 +
Sub into pBca1256         (EcoRI/BamHI 910+2472bp, L)                  
 +
Product is pBca1256-K112403
 +
----------------------------
 +
cb10007-phoA-F cgataGAATTCatgAGATCTgtacatggagaaaataaaatgaaacaaagc
 +
cb10008-phoA-R cgtagGGATCCgggcttttgtcacaggggtaaac
 +
</pre>
 +
 +
==pBca1256-K112404==
 +
<pre>
 +
Biobricking of signal peptide (prepro) of lamB protein
 +
PCR cb10009-F and cb10010 on MG1655 (132 bp, EcoRI/BamHI)
 +
Sub into pBca1256         (EcoRI/BamHI 910+2472bp, L)                  
 +
Product is pBca1256-K112404
 +
----------------------------
 +
cb10009-lamB-F cgataGAATTCatgAGATCTgaaaagcaatgactcaggagatag
 +
cb10010-lamB-R cgtagGGATCCcagccattgcctgagcagacattac
</pre>
</pre>

Revision as of 01:47, 2 July 2008

Contents

pBca1256-K112400

Biobricking of promoter for grpE protein 
 PCR grpE-F and grpE-R on MG1655 	  (129 bp, EcoRI/BamHI)
 Sub into pBca1256	                  (EcoRI/BamHI 910+2472bp, L)
 Product is pBca1256-K112400
 ----------------------------
cb0001-grpE-F Forward Biobricking    cgataGAATTCatgAGATCTccgaggtccttgttgcgaag
cb0002-grpE-R Reverse Biobricking    cgtagGGATCCcgcattcatctcgctaacttc

pBca1256-K112401

Biobricking of promoter for recA protein 
 PCR recA-F and recA-R on MG1655 	  (317 bp, EcoR1/BamH1)
 Sub into pBca1256	                  (EcoR1/BamH1 910+2472bp, L)
 Product is pBca1256-K112401
 ----------------------------
cb0003-recA-F Forward Biobricking    cgataGAATTCatgAGATCTagagaagcctgtcggcaccg
cb0004-recA-R Reverse Biobricking    cgtagGGATCCcccagtgctgccgccaacgc

pBca1256-K112402

Biobricking of promoter for fabA protein 
 PCR fabA-F and fabA-R on MG1655 	  (285 bp, EcoR1/BamH1)
 Sub into pBca1256	                  (EcoR1/BamH1 910+2472bp, L)
 Product is pBca1256-K112402
 ----------------------------
cb0005-fabA-F Forward Biobricking   cgataGAATTCatgAGATCTggccattacgttggctgaac 
cb0006-fabA-R Reverse Biobricking   cgtagGGATCCgaagttaccacccgtttcgg

pBca1256-K112403

Biobricking of signal peptide (prepro) of phoA (alkaline phosphatase) 
 PCR cb10007-F and cb10008 on MG1655	(114 bp, EcoRI/BamHI)
 Sub into pBca1256	        	(EcoRI/BamHI 910+2472bp, L)	                  
 Product is pBca1256-K112403
 ----------------------------
cb10007-phoA-F	cgataGAATTCatgAGATCTgtacatggagaaaataaaatgaaacaaagc
cb10008-phoA-R	cgtagGGATCCgggcttttgtcacaggggtaaac

pBca1256-K112404

Biobricking of signal peptide (prepro) of lamB protein 
 PCR cb10009-F and cb10010 on MG1655	(132 bp, EcoRI/BamHI)
 Sub into pBca1256	        	(EcoRI/BamHI 910+2472bp, L)	                  
 Product is pBca1256-K112404
 ----------------------------
cb10009-lamB-F 		cgataGAATTCatgAGATCTgaaaagcaatgactcaggagatag
cb10010-lamB-R 		cgtagGGATCCcagccattgcctgagcagacattac
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