Team:Chiba/Calendar-Home/22 August 2008
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#Incubated cultures(from Glycerol Stocks) with 2mL of LB-Ampicillin, Chloramphenicol Medium and LB-Ampicilline, Chloramphenicol and AHL100nm Medium for 12 hours at 37 degree. | #Incubated cultures(from Glycerol Stocks) with 2mL of LB-Ampicillin, Chloramphenicol Medium and LB-Ampicilline, Chloramphenicol and AHL100nm Medium for 12 hours at 37 degree. | ||
#moved the cultures to small plates and started UV-irradiation. (wavelength:254nm,distance from the UV lamp to the cultures were 8cm.) | #moved the cultures to small plates and started UV-irradiation. (wavelength:254nm,distance from the UV lamp to the cultures were 8cm.) | ||
- | #After exposing both plates to UV light for 15min, 4h, 8h, or 12h, we took 20ul from each plate, diluted 10 | + | #After exposing both plates to UV light for 15min, 4h, 8h, or 12h, we took 20ul from each plate, diluted 10<sup>5</sup>-fold, and plated 20ul of the resulting solution t (LB-Amp,Cm)(LB-Amp,cm,AHL100nM) plates. |
Revision as of 01:44, 30 October 2008
21 August 2008 <|> 23 August 2008
Contents |
Laboratory work
Team:Input
Mini prep: Minipreped the cultures(<--21/8).
- [http://partsregistry.org/Part:BBa_R0051 BBa_R0051](2007)
- [http://partsregistry.org/Part:BBa_R0051 BBa_R0051](2006)
- [http://partsregistry.org/Part:BBa_J06650 BBa_J06650](2007)
- [http://partsregistry.org/Part:BBa_J06650 BBa_J06650](2006)
- [http://partsregistry.org/Part:BBa_J22136 BBa_J22136](2007)
- [http://partsregistry.org/Part:BBa_J22141 BBa_J22141](2007)
insert check:Digestion
- [http://partsregistry.org/Part:BBa_J06650 BBa_J06650](2006)
- [http://partsregistry.org/Part:BBa_J06650 BBa_J06650](2007)
- [http://partsregistry.org/Part:BBa_J22136 BBa_J22136](2007)
- [http://partsregistry.org/Part:BBa_J22141 BBa_J22141](2007)
- [http://partsregistry.org/Part:BBa_R0051 BBa_R0051](2006)
- [http://partsregistry.org/Part:BBa_R0051 BBa_R0051](2007)
Sample No. | 1-4 | 1-6 |
dH2O(μl) | 14 | 25 |
10×BSA(μl) | 7 | 10 |
10×NE(μl) | 7 | 10 |
EcoRI(μl) | 3.5 | 5 |
PstI(μl) | 3.5 | - |
Total(μl) | 35 | 50 |
UV irradiation test
- two plasmids from
o -Ptrc-LuxR-Plux-cI-colE1-Amp- o -PcI-GFP-p15a-Cm-(JW1908)
- Incubated cultures(from Glycerol Stocks) with 2mL of LB-Ampicillin, Chloramphenicol Medium and LB-Ampicilline, Chloramphenicol and AHL100nm Medium for 12 hours at 37 degree.
- moved the cultures to small plates and started UV-irradiation. (wavelength:254nm,distance from the UV lamp to the cultures were 8cm.)
- After exposing both plates to UV light for 15min, 4h, 8h, or 12h, we took 20ul from each plate, diluted 105-fold, and plated 20ul of the resulting solution t (LB-Amp,Cm)(LB-Amp,cm,AHL100nM) plates.
The number of colonies aftetr culturing at 37C for 12h.
no AHL | AHL100nM | |
15min | 136 | 0 |
4h | 40 | 31 |
8h | 10 | 0 |
12h | 0 | 0 |
Colonies with no AHL plate observed GFP,
いづれのプレートもAHLが入っていないものはGFPが確認できたが、AHL100nMのものは確認できなかった。
Observe GFP fluorescence using UV exposure
Team:Communication
- Transformation
- competent cells : XL10G
- [http://partsregistry.org/Part:BBa_J13002 BBa_J13002](2007)
- [http://partsregistry.org/Part:BBa_J13002 BBa_J13002](2006)
- [http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007)
- [http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2006)
- (Locate & 2 Punch ~iyama ver.~)
- [http://partsregistry.org/Part:BBa_C0076 BBa_C0076](2008) -> No colonies on plates
- [http://partsregistry.org/Part:BBa_C0077 BBa_C0077](2008) -> No colonies on plates
- [http://partsregistry.org/Part:BBa_C0078 BBa_C0078](2008) -> No colonies on plates
- [http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2008) -> No colonies on plates
--->(24/8)Mini prep
Team:Output
- [http://partsregistry.org/Part:BBa_J52008 BBa_J52008]
- [http://partsregistry.org/Part:BBa_F2620 BBa_F2620]
- [http://partsregistry.org/Part:BBa_T9002 BBa_T9002]
- [http://partsregistry.org/Part:BBa_J33202 BBa_J33202]
- PUC19
- PGEX VENUS YFP(GST fusion)
- PGEX VENUS YFP(GST fusion)